Knenotransplantasyon Modelinin KanLarında Dolaşan Domuza Özgü DNA'nın Ölçülmesi
3.4K görüntülenme
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2 kez atıf aldı
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07:34 dk.
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22 Eylül 2020
DOI :10.3791/61579-v
22 Eylül 2020
•3.4K görüntülenme
Yangyang Deng*1,2 , Ming Zhou*1,3 , Ying Lu1 , Jiao Chen1 , Zuhui Pu4 , Dongjing Yu1,5 , Yifan Dai6 , Yongqiang Zhan7 , Lisha Mou1
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 2Xianning Hospital of Traditional Chinese Medicine, 3Liver-biotechnology (Shenzhen) Co., Ltd., 4Department of Radiology, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 5Department of Life Sciences, University of Toronto, 6Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 7Department of Hepatopancreatobiliary Surgery, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital
* These authors contributed equally
Bu protokolde, domuza özgü astarlar tasarlanmış, plazmid içeren domuz spesifik DNA parçaları inşa edilmiş ve nicellik için standart eğriler oluşturulmuştur. Türe özgü astarlar kullanılarak, domuzdan fareye hücre nakli modellerinde ve domuzdan maymuna arter yama transplantasyonu modellerinde cpsDNA qPCR ile ölçüldü.
Chapters in this video
0:04
Introduction
0:52
Isolating Genomic DNA
2:20
Verification of Primer Specificity
3:42
Standard Curve of cpsDNA
4:45
Quantitation of Circulating Pig-specific DNA
5:55
Results: PCR Validation of Porcine Specific Primers
6:51
Conclusion
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