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RNA-seq Verilerinde Alternatif Ekleme ve Poliadenilasyonun Tanımlanması
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
JoVE Journal
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JoVE Journal Biyoloji
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

RNA-seq Verilerinde Alternatif Ekleme ve Poliadenilasyonun Tanımlanması

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5,467 Views

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08:35 min

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June 24, 2021

DOI:

10.3791/62636-v

DOI:
10.3791/62636-v

•

08:35 min
June 24, 2021

•

5447 Views
, , ,
1Department of Genome Sciences, The John Curtin School of Medical Research,The Australian National University2Biyoloji Bölümü,New York University

Özet

Automatically generated

Alternatif ekleme (AS) ve alternatif poliadenilasyon (APA), transkript izoformlarının ve ürünlerinin çeşitliliğini genişletir. Burada, deneysel koşullar arasında değişen AS ve APA'yı tespit etmek ve görselleştirmek için toplu RNA-seq ve 3' uç dizileme testlerini analiz etmek için biyoinformatik protokolleri açıklıyoruz.

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Cite this Article

Dixit, G., Zheng, Y., Parker, B., Wen, J. Identification of Alternative Splicing and Polyadenylation in RNA-seq Data. J. Vis. Exp. (172), e62636, doi:10.3791/62636 (2021).

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