Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

Aym&#233; Oliva C&#225;rdenas; Blanca C. Zamora-Rodr&#237;guez; Kevin A. Batalla-Garc&#237;a; Alejandro &#193;valos-Rodr&#237;guez; Alejandra Contreras-Ramos; Clara Ortega-Camarillo

doi:10.3791/65172

Sprague Dawley Sıçanlarının Yağ Dokusundan Elde Edilen Mezenkimal Kök Hücrelerin İzolasyonu ve Ta...

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Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

Sprague Dawley Sıçanlarının Yağ Dokusundan Elde Edilen Mezenkimal Kök Hücrelerin İzolasyonu ve Tanımlanması

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10:50 min

April 7, 2023

DOI: 10.3791/65172-v

Aymé Oliva Cárdenas^1,2, Blanca C. Zamora-Rodríguez², Kevin A. Batalla-García², Alejandro Ávalos-Rodríguez³, Alejandra Contreras-Ramos⁴, Clara Ortega-Camarillo²

¹Doctorado en Ciencias Biológicas y de la Salud,Universidad Autónoma Metropolitana, ²Medical Research Unit in Biochemistry, Specialties Hospital, National Medical Center SXXI,Instituto Mexicano de Seguro Social, ³Department of Agricultural and Animal Production, Division of Biological and Health Science,Universidad Autónoma Metropolitana-Xochimilco, ⁴Molecular Biology Research Laboratory, Center for Congenital Malformations,Children Hospital of Mexico Federico Gomez (HIMFG)

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Overview

This study presents a protocol for isolating and characterizing adipose tissue-derived mesenchymal stem cells (MSCs) from Sprague Dawley rats. These MSCs possess significant potential for application in therapies aimed at pancreatic regeneration and the treatment of diabetes.

Key Study Components

Research Area

Mesenchymal stem cells
Cell therapies for diabetes
Pancreatic tissue regeneration

Background

The role of MSCs in cellular communication
Paracrine functions of stem cells
Current challenges in cellular therapy for diabetes

Methods Used

Isolation and identification of adipose-derived mesenchymal stem cells
Use of Sprague Dawley rats
Functional assessments of stem cells

Main Results

Successful isolation of MSCs with expected morphological and immunophenotypical characteristics
Utilization of stem cells for evaluating diabetes-related therapies
Contribution of miRNAs to pancreatic tissue degeneration

Conclusions

The study confirms the potential of adipose MSCs in cellular therapy for pancreatic diseases.
This research enhances understanding of diabetes treatment strategies using stem cells.

Frequently Asked Questions

What are mesenchymal stem cells?

Mesenchymal stem cells are multipotent stem cells capable of differentiating into various cell types and are known for their roles in healing and tissue regeneration.

Why use adipose tissue for isolating stem cells?

Adipose tissue is an abundant source of mesenchymal stem cells and is relatively easier to procure than other stem cell sources.

How do MSCs contribute to diabetes therapies?

MSCs can participate in tissue repair and regeneration processes, which may help to restore insulin production and improve glucose metabolism in diabetic patients.

What role do miRNAs play in this research?

miRNAs are investigated for their contributions to pancreatic tissue degeneration, particularly under oxidative stress conditions.

What is the significance of paracrine function in MSCs?

Paracrine signaling allows MSCs to communicate with other cells, influencing their behavior and promoting repair processes.

What technologies are used in this study?

Techniques like microarrays and next-generation sequencing are employed to study the functions of miRNAs and their impact on cellular processes.

What are the implications of this research?

This research provides insight into enhancing cell therapy strategies for diabetes, ultimately aiming to improve patient outcomes through regenerative medicine.

Bu protokol, Sprague Dawley sıçanlarından yağ dokusu kaynaklı mezenkimal kök hücrelerin (MSC'ler) izole edilmesi ve tanımlanması için bir metodoloji açıklamaktadır.

Mezenkimal kök hücreler, parakrin fonksiyonları nedeniyle hücresel iletişimde gerçekten geniş bir role sahiptir. Bu protokol, pankreas rejenerasyonu ve diyabet tedavilerinin incelenmesinde kullanılmak üzere morfolojik, immünofenotipik ve yeterli işlevselliğe sahip adipoz kaynaklı mezenkimal kök hücreler elde eder. Diyabet tedavisinde hücre tedavilerinin kullanımı artmıştır.

Bu tedaviler, pankreas hücrelerinin allojenik nakillerini, insülin üreten hücrelerin otolog kök hücre türevi ürünlerini ve biyolojik mürekkepler kullanılarak 3D baskılı pankreas dokusunu içerir. Çoğu araştırma öncelikle sekretom bileşenlerini ve farklı hücresel süreçlerin düzenlenmesindeki rolünü inceledi. Mikrodiziler ve yeni nesil dizileme gibi yeni teknikler miRNA'ların işlevini araştırabilir.

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