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Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
JoVE Journal
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JoVE Journal Biyomühendislik
Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth

808 Views

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02:33 min

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May 17, 2024

DOI:

10.3791/65767-v

DOI:
10.3791/65767-v

•

02:33 min
May 17, 2024

•

27 Views
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1Department of Dentistry, Oral Health Institute,Hamad Medical Corporation, 2Pushpagiri Institute of Medical Sciences and Research Centre, 3Saveetha Dental College,Saveetha Institute of Medical and Technical Sciences, 4College of Applied Medical Sciences,King Saud University

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This protocol emphasizes the extraction, culture, and preservation of multipotent stem cells from dental pulp through enzymatic digestion. Additionally, it demonstrates their potential to differentiate into osteoblasts, adipocytes, and chondrocytes, highlighting the importance of precision and consistency in the process.

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Dental Pulp Stem CellsDPSCsDeciduous TeethPermanent TeethRegenerative MedicineMesenchymal Stem CellsEnzymatic DigestionOutgrowthAdipocytesOsteoblastsChondrocytesMultipotentDifferentiationCell IsolationCell CultureCell Preservation

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Cite this Article

Anil, S., Thomas, N. G., Chalisserry, E. P., Dalvi, Y. B., Ramadoss, R., Vellappally, S. Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth. J. Vis. Exp. (207), e65767, doi:10.3791/65767 (2024).

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