Name: Periadventitial Kalsiyum Klorür ve Elastaz İnfiltrasyonu ile Bir Fare Abdominal Aort Anevrizması Modeli (Video) | JoVE Journal
Uploaded: 2024-08-02T13:40:00.000+00:00
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Description: Öğrenin, farelerde abdominal aort anevrizması modeli oluşturmayı kalsiyum klorür ve elastaz kullanarak. Bu güvenli ve tekrarlanabilir yöntem, insanlardaki patofizyolojik değişiklikleri taklit ederek vasküler biyoloji araştırmalarına katkı sağlar.

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<meta charset="utf8"></head><body>Murin abdominal aort anevrizmasının altında yatan moleküler mekanizmaların araştırılması

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</ol>

<table><tbody><tr><td>Anestezi Makinesi</td><td>RWD</td><td>R550</td><td></td></tr><tr><td>Butorphanol</td><td>Jiangsu Hengrui Pharmaceutical Co., Ltd</td><td>220608BP</td><td>1ml: 1mg</td></tr><tr><td>C57BL/6JGpt Erkek Fareler</td><td>GemPharmatech</td><td>N000013</td><td></td></tr><tr><td>Kalsiyum Klorür</td><td>Sigma Aldrich</td><td>C4901</td><td>100 g</td></tr><tr><td>Carprofen</td><td>MCE</td><td>HY-B1227</td><td>100 mg</td></tr><tr><td>Chow Diyeti</td><td>Dossy Deney Hayvanları Co.Ltd</td><td></td><td></td></tr><tr><td>Dijital Kumpas</td><td>Greener</td><td>IP54</td><td></td></tr><tr><td>Etanol Jinhe</td><td>İlaç Co.Ltd</td><td>539682</td><td>%75/500 mL</td></tr><tr><td>EVG Boyama Kiti</td><td>Solarbio</td><td>G1597</td><td></td></tr><tr><td>GraphPad Prizma</td><td>Graphpad</td><td></td><td>Ver 9.0.0</td></tr><tr><td>H & E Boyama Kiti</td><td>Servicebio</td><td>G1076</td><td></td></tr><tr><td>İnsülin Şırıngası</td><td>BD</td><td>2143420</td><td></td></tr><tr><td>İzofluran</td><td>RWD</td><td>R510-22-4</td><td>100 mL</td></tr><tr><td>Masson Boyama Kiti</td><td>Servicebio</td><td>G1006</td><td></td></tr><tr><td>Normal Salin</td><td>Servicebio</td><td>G4702</td><td>500 mL</td></tr><tr><td>Paraformaldehit</td><td>Biosharp</td><td>BL539A</td><td>%4/500 mL</td></tr><tr><td>PBS, 1x</td><td>Servicebio</td><td>G4202</td><td>500 mL</td></tr><tr><td>Domuz Pankreas Elastaz</td><td>Sigma Aldrich</td><td>E1250</td><td>100 mg</td></tr><tr><td>Povidin İyot</td><td>Yongan İlaç Co.Ltd</td><td></td><td>%5/100 mg</td></tr><tr><td>Prolene Polipropilen Sütür</td><td>Ethicon LLC</td><td>8709H</td><td></td></tr><tr><td>Kemirgen Ultrason Sistemi</td><td>Fujifilm</td><td>Vevo 3100LT</td><td></td></tr><tr><td>Stereo Mikroskop</td><td>Olympus</td><td>SZ61</td><td></td></tr><tr><td>Ultrasonik Kuplant Küpler</td><td></td><td>KL-250</td><td>250 g</td></tr></tbody></table>

periadventitial kalsiyum klorür ve elastaz İnfiltrasyonu ile bir fare abdominal aort anevrizması modeli

A Mouse Abdominal Aortic Aneurysm Model by Periadventitial Calcium Chloride and Elastase Infiltration

Bu çalışmada, toplam 24 fare dahil edildi ve rastgele atandı: sırasıyla Sham grubunda 12 ve PPE + CaCl2 grubunda 12. Tüm veriler, aksi belirtilmedikçe ortalama ± standart sapmalar olarak sunulur. Ortalama ameliyat süresi 55.67 dk ± 4.08 dk idi. İntraoperatif ölüm veya anevrizma rüptürü olmadı ve ameliyattan sonraki 21 gün içinde hayatta kalma oranı 0 idi. Abdominal aort diseksiyonuna bağlı ciddi intestinal yapışıklıklar veya komplikasyonlar gözlenmedi.
PPE + CaCl2 grubunda, 12 fareden 11'inde (% 91.67) ameliyattan 21 gün sonra AAA gelişirken, Sham grubunda anevrizma oluşumu gözlenmedi. İnfrarenal abdominal aortun ortalama maksimum iç çapı PPE + CaCl2 grubunda 1.29 mm ± 0.18 mm iken, Sham grubunda 0.50 mm ± 0.04 mm idi. PPE + CaCl2&amp;E<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/66674/66674fig01.jpg"> &amp;<a href="https://www.jove.com/files/ftp_upload/66674/66674fig01large.jpg" target="_blank"></a>

Periadventitial Kalsiyum Klorür ve Elastaz İnfiltrasyonu ile Bir Fare Abdominal Aort Anevrizması Modeli

Abdominal aortic aneurysm (AAA) is a life-threatening disease associated with high mortality rates. It is characterized by the permanent dilation of the ...

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Tıp

Bu video infrarenal abdominal aort segmentine domuz pankreatik elastaz geçici intraluminal infüzyonu ile farelerde abdominal aort anevrizması (AAA) ikna etmek için nasıl gösterir. Modeli birçok transgenik ve gen knockout farelerde ortaya çıkması nedeniyle AAA patobiyolojisi geniş bir fikir eklemek için yeteneğine sahiptir.

Elastaz ile murin Deneysel Abdominal Aort Anevrizmalarının Yaratılış

Mevcut protokol, farelerde infrarenal abdominal aortun adventitisine doğrudan elastaz uygulanması yoluyla elastaz kaynaklı AAA modeli için standartlaştırılmış bir cerrahi yöntemi tanımlamaktadır.

Elastazın Perivasküler Uygulaması ile İndüklenen Fare Abdominal Aort Anevrizması Modeli

Bu protokol, AAA'ların patolojik özelliklerini ve moleküler mekanizmalarını incelemek için kalsiyum fosfata bağlı abdominal aort anevrizması (AAA) fare modelini tanımlamaktadır.

Kalsiyum Fosfat Kaynaklı Fare Abdominal Aort Anevrizması Modeli

Bu protokol, farelerde ileri abdominal aort anevrizmalarının modellenmesine yönelik metodik bir cerrahi yaklaşımı, infrarenal aorta doğrudan elastaz uygulanması ve içme suyu yoluyla ß-aminopropionitrilin uygulanmasının bir kombinasyonu ile tanımlar.

Topikal Elastaz ve Oral ß-aminopropionitril kombinasyonu ile farelerde ileri abdominal aort anevrizması modellemesi

Bu protokol, elastin hassasiyeti ve perivasküler elastaz uygulaması kullanarak C57BL/6 farelerinde abdominal aort anevrizmalarını (AAA) indüklemek için tekrarlanabilir ve güvenilir bir yöntem sunar; bu yöntem bağışıklık duyarlılığı bileşeni içeren bir AAA modelidir.

Elastin duyarlılığı ve perivasküler elastaz kullanarak güvenilir bir fare karın aort anevrizması modeli oluşturulması

Anevrizma endogreft dışlama sonra histolojik ve biyokimyasal değişiklikler hala belirsiz. Bir fare anevrizma üzerinde endogreft implantasyon yeni bir model tarif. Kalıcı dolaşan kan akışı arayüzü ve yeni endovasküler biyomalzemeler değerlendirme olan ve olmayan trombüs analizi sayesinde, bu model daha iyi endovasküler anevrizma dışlama patobiyolojisi anlamanıza yardımcı olacaktır.

Endovasküler Aort Anevrizma Onarımı Yeni Bir murin Modeli

Farelerde FeCl3 ile indüklenen tromboz modeli burada açıklanmıştır. Mezenterik bir damarda intravital mikroskopi gözlemi ve karotis arterde kan akışı ölçümü ile trombüs büyümesini izlemek için bir yöntem sunulmaktadır.

Ferric Klorür kaynaklı Karotis Arter ve Mezenter Gemi Tromboz Fare Modeli

arteriyel denüdasyon yaralanmasının ardından yeniden endotelizas- hücresel ve moleküler mekanizmaların anlaşılması trombozu ve arterlerin restenoz önlemede büyük önem taşımaktadır. Burada İnfrarenal abdominal aorta tekrarlanabilir arter denüdasyon yaralanması için bir protokol açıklar. prosedür fare modelleri kullanılarak endotel rejenerasyon düzenleyen altında yatan mekanizmaları araştırmak için geliştirilmiştir.

Bir Fare Modelinde İnfrarenal Abdominal Aort Bağlama Ekipmanları tarafından tekrarlanabilir Arter denüdasyon Yaralanma

Biz farelerde kol tekniği kullanarak bir orthotopic aort nakli modeli mevcut. Bu damar hastalığı çalışmalarda istihdam bir çok hızlı anastomoz yöntemdir.

Kol tekniği aort transplantasyonu - eğitici bir Video bir fare Model kullanma

Aort Lümen ve dikiş ligasyonu aort içine poliüretan kateter ekleme neden adventitial vasa vasorum hipoperfüzyon nedeniyle kronik hipoksi. Bu makalede, abdominal aort anevrizması (AAA) insanlarda bu AAA benzer özelliklere sahip yeni bir hayvan modeli.

Abdominal aort anevrizması Adventitial Vasa Vasorum perfüzyon engelleyerek kemirgen bir Model oluşturulması

Öğrenin, farelerde abdominal aort anevrizması modeli oluşturmayı kalsiyum klorür ve elastaz kullanarak. Bu güvenli ve tekrarlanabilir yöntem, insanlardaki patofizyolojik değişiklikleri taklit ederek vasküler biyoloji araştırmalarına katkı sağlar.

Periadventitial Kalsiyum Klorür ve Elastaz İnfiltrasyonu ile Bir Fare Abdominal Aort Anevrizması Modeli

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