Tekrarlayan İmplantasyon Başarısızlığı Olan Kadınlarda Çoklanmış Floresan İmmünohistokimyasal Boyama Kullanılarak Dört Uterin NK Hücre Alt Tipinin Ölçümü

2.2K görüntüleme

2 kez atıf

08:29 dk

25 Ekim 2024

10.3791/67284-v

25 Ekim 2024

2.2K görüntüleme

Lu Chen¹ , Loucia Kit Ying Chan¹ , Stacey Chui Yiu Wong¹ , Joshua Jing Xi Li² , Jacqueline Pui Wah Chung¹ , Chi Chiu Wang¹^,³^,⁴^,⁵ , Mingqing Li⁶^,⁷^,⁸ , Tao Zhang¹

¹Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, ²Department of Pathology, School of Clinical Medicine, The University of Hong Kong, Queen Mary Hospital, ³Reproduction and Development Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, ⁴School of Biomedical Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, ⁵Chinese University of Hong Kong - Sichuan University Joint Laboratory in Reproductive Medicine, The Chinese University of Hong Kong, ⁶Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University, ⁷NHC Key Lab of Reproduction Regulation, Shanghai Institute for Biomedical and Pharmaceutical Technologies, Fudan University, ⁸Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Hospital of Obstetrics and Gynecology, Fudan University

Bu çalışma, ileri çoğullanmış floresan immünohistokimyasal boyama teknikleri kullanılarak implantasyon penceresi sırasında uterus doğal öldürücü hücre alt kümelerini ölçmek için öncü bir yöntem sunmaktadır.

Daha fazla video keşfet

Uterin NK H creleri

Multiplex mm nohistokimya

mm n H cre Alt Tipleri

Chapters in this video

0:00

Introduction

1:35

Multiplex Immunohistochemistry for Uterine NK Cell Subtype Analysis in Endometrial Tissues

4:26

Image Acquisition After Multiplex Immuno‐Staining for Distinguishing uNK Cell Subtypes in Endometrial Tissues

5:51

Image Analysis to Characterize uNK Cell Subtypes in Early Pregnancy Using Multiplex Immunohistochemistry