Name: Gelişmekte Olan ve Olgun Arabidopsis Tohumlarında Endosperm Hücre Tabakasının Mikroskobik Analizi için Sağlam Dokunun Hazırlanması (Video) | JoVE Journal
Uploaded: 2025-05-16T14:40:00.000+00:00
Duration: 6 min 28 s
Description: Keşfedin, Arabidopsis thaliana tohumlarında endosperm hücre tabakalarının canlı hücre görüntülemesi için sağlam doku hazırlama protokolünü. Bu yöntem, mitokondri dinamiği ve hücresel süreçleri yüksek çözünürlükle incelemenizi sağlar.

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<li>Xu, G., Zhang, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mechanisms+controlling+seed+size+by+early+endosperm+development%5BTitle%5D)+AND+%22Seed+Biol%22%5BJournal%5D)">Mechanisms controlling seed size by early endosperm development.</a> Seed Biol. 2, 1(2023).</li>
</ol>

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gelişmekte olan ve olgun arabidopsis tohumlarında endosperm hücre tabakasının mikroskobik analizi için sağlam dokunun hazırlanması

Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds

Şekil 1'de gösterilen protokol kullanılarak, endosperm örnekleri, 14 DAF'ta siliklerden hasat edilen gelişmekte olan tohumlardan hazırlandı (bu aşamada, testa hala yeşildir). Geniş bir alanda çok sayıda endosperm hücresi ve bunların hücre içi yapıları gözlendi (Şekil 3A). Bu deneyde, C-terminalinde (PHYB-GFP) GFP ile kaynaşmış PHYB eksprese eden tohumlar kullanılmıştır. PHYB'nin kırmızı ışıkla aktivasyon üzerine çekirdeğe yer değiştirdiği ve tohum çimlenmesinde ...

Gelişmekte Olan ve Olgun Arabidopsis Tohumlarında Endosperm Hücre Tabakasının Mikroskobik Analizi için Sağlam Dokunun Hazırlanması

In Arabidopsis seeds, the endosperm, a single layer of living cells located between the embryo and the testa, plays a critical role in regulating seed ...

preparation-intact-tissue-for-microscopic-analysis-endosperm-cell

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Basma, bitki ve memeli üreme bir olgudur. DNA metilasyon, imprinting mekanizmaları önemli bir rol oynar. Endosperm Yalıtımlı ve baskılı genlerinin metilasyon durumunun...

Baskılı Genler DNA metilasyon Belirlenmesi Arabidopsis Endosperm

Biz stoma farklılaşma belgeleyen, gelişme birkaç gün içinde epidermis konfokal görüntüleme için bitki kotiledon hareketsiz odası slaytlar ve medya kullanarak bir protokol açıklar. Fluorofor etiketli proteinlerin hücre bölünmesi ve hücre tipi farklılaşması esnasında olası rollerini anlamayı arttırıyor, ifade ve hücre içi lokalizasyonu dinamik izlenebilir.

Uzun süreli, yüksek çözünürlüklü Konfokal Time Lapse Görüntüleme<em&gt; Arabidopsis Kotiledon</emÇimlenme sırasında&gt; Epidermis

Biz gelen geliştirmenin erken aşamalarında embriyo izole için etkili ve basit bir yöntem...

Erken evre Embriyolar verimli ve hızlı İzolasyon<em&gt; Arabidopsis thaliana</em&gt; Tohumlar

Biz Arabidopsis thaliana tohum tohum kat yatak tahlili (SCBA) monte prosedürü sunuyoruz. SCBA genetik ve in vitro keşfetmek için güçlü bir araç olduğu gösterilmiştir nasıl uyuyan tohum ve hafif uyaranlara yanıt olarak endosperm kontrolleri tohum çimlenme. SCBA endosperm embriyonik büyümeyi etkilemek için şüphelenilen her durumda altında uygulanabilir prensip olarak.

Genetiği keşfedin A Tohum Kabuğunun Yatak Deneyi<em&gt; In Vitro</em&gt; Nasıl Endosperminde Denetimler Çimlenme içinde<em&gt; Arabidopsis thaliana</em

Biz İmmunosteyn, floresan in situ hibridizasyon, tüm montaj Arabidopsis thaliana ovüllerindeki kantitatif, yüksek çözünürlüklü görüntüleme ile takip DNA boyama için burada etkin ve güvenilir bir protokol sağlar. Bu yöntem başarıyla kromatin modifikasyonları ve nükleer mimarisi kullanılarak analiz edildi.

Tüm montaj ovüllerindeki in Kromatin Modifikasyonu ve Nükleer Mimarlık Kantitatif, Tek hücreli Analizi için Etkin Bir Yöntem<em&gt; Arabidopsis</em

Kristalin selüloz, bitki hücre duvarının önemli bir bileşenidir. Bununla birlikte, hücresel bir çözünürlükte nicelleştirilmesi teknik olarak zordur.  Burada, uzay-zamansal bir çözünürlükte hücre duvarı bileşimi hakkında bilgi elde etmek için polarize ışık teknolojisinin ve kök kesitlerinin kullanımını rapor ediyoruz.

Kristalin Selüloz Birikimin Yüksek Çözünürlüklü Niceleme<em&gt; Arabidopsis</em&gt; Kökler Doku spesifik Hücre Duvarı Değişiklikler Monitör

Bu iletişim kuralı Arabidopsis içinde embriyogenez embriyo Desen oluşumu bir mikroskop altında incelenmesi ve ardından ovule izni ile gözlemlemek için bir yöntem özetliyor.

Embriyogenez Arabidopsis olarak karakterize bir yöntem

Bu iletişim kuralı, biz teknikleri için uygun diseksiyon Arabidopsis çiçek ve siliques, bazı temel Temizleme teknikleri tarif ve yordamlar yapılar gözlemleri bütün-mount için boyama seçili.

Bütün Dağı takas ve Arabidopsis çiçek organ ve Siliques boyama

Bu teknik, tohumun farklı bölgelerindeki hücrelerin, nişasta granüllerinin ve protein gövdelerinin gözlem ve analizi için tam tohum büyüklüğünde reçine bölümünün hızlı ve basit hazırlanmasına olanak sağlar.

Tam Tohum Lu Reişin Elde Edilmesinde Basit Bir Kuru Kesit Yöntemi ve Uygulamaları

Burada, Arabidopsis thaliana'da polen tüpü rehberliğini ve alımını gözlemlemek için ovüllerin alıcılığını artıran yarı in vitro (SIV) yöntemin geliştirilmesini açıklıyoruz. Yüksek verimli SIV cum septum yöntemi, dinamik döllenme sürecini izlemek için gametofit işaretleyici çizgileri ve genetik olarak kodlanmış biyosensörlerle birleştirilebilir.

Arabidopsis Polen Tüpü Alımı ve Çift Döllenmenin Semi-In Vitro Cum Septum Yöntemi Kullanılarak Canlı Görüntülenmesi

Keşfedin, Arabidopsis thaliana tohumlarında endosperm hücre tabakalarının canlı hücre görüntülemesi için sağlam doku hazırlama protokolünü. Bu yöntem, mitokondri dinamiği ve hücresel süreçleri yüksek çözünürlükle incelemenizi sağlar.

Gelişmekte Olan ve Olgun Arabidopsis Tohumlarında Endosperm Hücre Tabakasının Mikroskobik Analizi için Sağlam Dokunun Hazırlanması (Video) | JoVE Journal

Gelişmekte Olan ve Olgun Arabidopsis Tohumlarında Endosperm Hücre Tabakasının Mikroskobik Analizi için Sağlam Dokunun Hazırlanması

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