Name: Retinal Ganglion Hücrelerinin Anatomik Özelliklerinin N-metil-D-aspartik Asit (NMDA) ile İndüklenen Eksitotoksisite Altında İncelenmesi (Video) | JoVE Journal
Uploaded: 2025-09-19T12:40:00.000+00:00
Duration: 7 min 11 s
Description: Keşfedin, fare retinasında NMDA kaynaklı eksitotoksisite altında retinal ganglion hücrelerinin anatomik özelliklerini ve yeni fenotiplerini genetik alkalen fosfataz etiketleme yöntemiyle inceleyin.

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</ol>

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retinal ganglion hücrelerinin anatomik Özelliklerinin n-metil-d-aspartik asit (nmda) ile İndüklenen eksitotoksisite altında İncelenmesi

Examination of Anatomical Features of Retinal Ganglion Cells Under N-methyl-D-aspartic Acid (NMDA)-induced Excitotoxicity

Vitreusta NMDA uygulaması, geleneksel olarak histolojik boyama veya RGC belirteçleri ile immünofloresan (IF) boyama kullanılarak tespit edilen RGC ölümüne neden olur. Deneysel ortamımızda, bir pan-RGC belirteci RBPMS ile geleneksel IF stratejisini kullanarak, NMDA enjeksiyonundan 1 hafta sonra RGC sayısının yaklaşık e oranında azaldığını doğruladık (Şekil 2E-G). Önceki çalışmalar, Tbr2 eksprese eden RGC'lerin gerçekten de ipRGC'ler34,35<sup...

Retinal Ganglion Hücrelerinin Anatomik Özelliklerinin N-metil-D-aspartik Asit (NMDA) ile İndüklenen Eksitotoksisite Altında İncelenmesi

Retinal ganglion cells (RGCs) are the output neurons of the retina, responsible for transmitting visual information to the brain. In the mature mouse ...

examination-anatomical-features-retinal-ganglion-cells-under-n-methyl

Research

JoVE Journal

Biology

Biyoloji

Intravitreal enjeksiyon, göz hastalıkları veya lokal tedavi doğrudan uygulama olarak görsel bilimler araştırma yaygın olarak kullanılan bir tekniktir. Bu video cam pipet yardımıyla 1 ml şırınga kullanarak intravitreal enjeksiyon için bir protokol gösterdi. Masif kanama ve lens hasarı önlemek konusunda yararlı ipuçları verilmiştir.

Oküler Hastalıkları Model kurulması için intravitreal enjeksiyon

Bu video retina ganglion hücrelerinin sayısı (RGCs) ve niceleme RGCs boyutları ölçülerek, iç nükleer tabaka kalınlığı ölçümü de retinanın morfometrik analizler, üç tip gösterir. Tekniği morfometrik analizler için basit ama bilimsel bir platform sunabilir.

Retina Bölüm morfometrik Analizleri

Bu video naif fare görsel projeksiyon kontrastlı MR görüntüleme ve akut optik sinir ezilme yaralanma ve kronik optik sinir dejenerasyonu ile ilişkili optik sinir dejenerasyonu tekrarlayan ve uzunlamasına in vivo çalışmalar için, bir klinik 3 T tarayıcı kullanarak, bir yöntemi göstermektedir knock-out fareler (p50 KO).

Farelerde kontrastlı MR ile optik sinir lifi Dürüstlük İn vivo

İmmünohistokimya protokolleri retinada, spesifik bir proteinin lokalizasyonu incelemek için kullanılır. Kalsiyum görüntüleme teknikleri retina ganglion hücrelerinin ve bunların akson kalsiyum dinamiklerini incelemek için istihdam edilmektedir.

Wholemount Sıçan Retina Immunohistochemical ve Kalsiyum Görüntüleme Yöntemleri

Bu iletişim kuralı yalıtma ve birincil fare serebral granül neurons (CGNs) 6-7 gün eski pups, CGNs kaybı için verimli iletim ve kazanç işlevi çalışmalar, kültür ve nöronal excitotoxicity NMDA kaynaklı modelleme için basit bir yöntem açıklar, düşük potasyum indüklenen hücre ölümü, DNA hasarı ve oksidatif stres aynı kültür modelini kullanarak.

Modelleme nöronal ölüm ve fare birincil serebellar granül nöronlar dejenerasyon

NMDA reseptörleri (NMDAR) çalışma daha büyük ölçekli, kolaylaştırmak ve küçük moleküller düzenleyici etkileri muayene ve tedavi uygulamaları izin vermek için bu iletişim kuralını hedefidir.

NMDA reseptörleri glisin/D-serin ve glutamat duyarlılık ile çalışmak için bir yüksek-den geçerek kalsiyum-flux tahlil

İn vivo görüntüleme, sağlık ve hastalıkta biyoloji çalışmaları için güçlü bir araçtır. Bu protokol, standart iki foton mikroskobu ile fare retinasının transpupiller görüntülemesini tanımlar. Ayrıca, retinanın birden fazla hücresel kohortunu floresan olarak etiketlemek için farklı in vivoimaging yöntemleri göstermektedir.

Transpupiller İki Foton Fare Retinasının In vivo Görüntülenmesi

Bu makalede, konfokal canlı mikroskopi kullanılarak primer hipokampal ve kortikal nöronlarda bazal redoks durumundaki farklılıkları ve akut pertürbasyonlara redoks yanıtlarını belirlemek için bir protokol açıklanmaktadır. Protokol, diğer hücre tiplerine ve mikroskoplara minimum değişiklikle uygulanabilir.

Primer Nöronlarda Mitokondriyal Glutatyon Redoksu Durumunun Oranmetrik Gösterge Kullanılarak Canlı Görüntülenmesi

Metanol, retinal ganglion hücrelerinin araştırılması için yararlı olan retinal tüm montaj preparatları ve uzun süreli depolama için yardımcı bir sabit ortam olarak kullanılabilir.

Retinal ganglion hücrelerinin araştırılması için metanol bazlı tam montajlı preparat

Bu video floro-altın (FG) üstün colliculus (SC) yüzeyine uygulanarak RGC retrograd etiketleme yöntem açıklanır. Tekniği, kafatası delme, korteks aspire ve SC tüm dorsal yüzey üzerinde jelatin sünger uygulayarak içerir.

Üstün Colliculus Yüzey Fluoro-Gold Uygulama Retina Ganglion Hücreleri Retrograd Etiketleme

Keşfedin, fare retinasında NMDA kaynaklı eksitotoksisite altında retinal ganglion hücrelerinin anatomik özelliklerini ve yeni fenotiplerini genetik alkalen fosfataz etiketleme yöntemiyle inceleyin.

Retinal Ganglion Hücrelerinin Anatomik Özelliklerinin N-metil-D-aspartik Asit (NMDA) ile İndüklenen Eksitotoksisite Altında İncelenmesi (Video) | JoVE Journal

Retinal Ganglion Hücrelerinin Anatomik Özelliklerinin N-metil-D-aspartik Asit (NMDA) ile İndüklenen Eksitotoksisite Altında İncelenmesi

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