Name: Sabit Yük (İzotonik) ve Sabit Hız (İzokinetik) Tork-Hız-Güç Profillerinin Oluşturulması Sıçan Plantar Fleksörleri için İn vivo (Video) | JoVE Journal
Uploaded: 2025-10-03T13:50:00.000+00:00
Duration: 7 min 44 s
Description: Öğrenin sıçan plantar fleksörlerinde izotonik ve izokinetik tork-hız-güç profillerini in vivo ölçme yöntemini. Bu protokol kas mekaniğini ve hastalıkların kas fonksiyonuna etkisini anlamak için kullanılır.

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<li>Hinks, A., Power, G. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Age-related+differences+in+the+loss+and+recovery+of+serial+sarcomere+number+following+disuse+atrophy+in+rats%5BTitle%5D)+AND+%22Skeletal+Muscle%22%5BJournal%5D)">Age-related differences in the loss and recovery of serial sarcomere number following disuse atrophy in rats.</a> Skeletal Muscle. 14 (1), 18(2024).</li>
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<li>Wang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Human+neural+stem+cells+transplanted+during+the+sequelae+phase+alleviate+motor+deficits+in+a+rat+model+of+cerebral+palsy%5BTitle%5D)+AND+%22Cytotherapy%22%5BJournal%5D)">Human neural stem cells transplanted during the sequelae phase alleviate motor deficits in a rat model of cerebral palsy.</a> Cytotherapy. 26 (12), 1491-1504 (2024).</li>
<li>Baltgalvis, K. A., Call, J. A., Cochrane, G. D., Laker, R. C., Yan, Z., Lowe, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Exercise+training+improves+plantar+flexor+muscle+function+in+mdx+mice%5BTitle%5D)+AND+%22Med+Sci+Sports+Exerc%22%5BJournal%5D)">Exercise training improves plantar flexor muscle function in mdx mice.</a> Med Sci Sports Exerc. 44 (9), 1671-1679 (2012).</li>
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<li>Song, H., Nakazato, K., Nakajima, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effect+of+increased+excursion+of+the+ankle+on+the+severity+of+acute+eccentric+contraction-induced+strain+injury+in+the+gastrocnemius%3A+an+in+vivo+rat+study%5BTitle%5D)+AND+%22Am+J+Sports+Med%22%5BJournal%5D)">Effect of increased excursion of the ankle on the severity of acute eccentric contraction-induced strain injury in the gastrocnemius: an in vivo rat study.</a> Am J Sports Med. 32 (5), 1263-1269 (2004).</li>
<li>Hinks, A., Patterson, M. A., Njai, B. S., Power, G. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Age-related+blunting+of+serial+sarcomerogenesis+and+mechanical+adaptations+following+4+weeks+of+maximal+eccentric+resistance+training%5BTitle%5D)+AND+%22J+Appl+Physiol%22%5BJournal%5D)">Age-related blunting of serial sarcomerogenesis and mechanical adaptations following 4 weeks of maximal eccentric resistance training.</a> J Appl Physiol. 136 (5), 1209-1225 (2024).</li>
<li>Hinks, A. A., Vlemmix, E., Power, G. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Submaximal+eccentric+resistance+training+increases+serial+sarcomere+number+and+improves+dynamic+muscle+performance+in+old+rats%5BTitle%5D)+AND+%22Physiol+Rep%22%5BJournal%5D)">Submaximal eccentric resistance training increases serial sarcomere number and improves dynamic muscle performance in old rats.</a> Physiol Rep. 12 (19), e70036(2024).</li>
<li>Hinks, A., Vlemmix, E., Power, G. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Submaximal+eccentric+training+during+immobilization+does+not+prevent+serial+sarcomere+loss+or+impairments+in+mechanical+function+in+old+or+young+rats%5BTitle%5D)+AND+%22J+Gerontol+A+Biol+Sci+Med+Sci%22%5BJournal%5D)">Submaximal eccentric training during immobilization does not prevent serial sarcomere loss or impairments in mechanical function in old or young rats.</a> J Gerontol A Biol Sci Med Sci. 80 (7), glaf082(2025).</li>
<li>Vlemmix, E., Hinks, A., Power, G. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Serial+sarcomerogenesis+does+not+contribute+to+the+initial+repeated+bout+effect+in+skeletal+muscle%5BTitle%5D)+AND+%22J+Biomech%22%5BJournal%5D)">Serial sarcomerogenesis does not contribute to the initial repeated bout effect in skeletal muscle.</a> J Biomech. 187, 112767(2025).</li>
<li>Oh, S. S., Narver, H. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mouse+and+rat+anesthesia+and+analgesia%5BTitle%5D)+AND+%22Curr+Protoc%22%5BJournal%5D)">Mouse and rat anesthesia and analgesia.</a> Curr Protoc. 4 (2), e995(2024).</li>
<li>Paris, M. T., McNeil, C. J., Power, G. A., Rice, C. L., Dalton, B. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Age-related+performance+fatigability%3A+a+comprehensive+review+of+dynamic+tasks%5BTitle%5D)+AND+%22J+Appl+Physiol%22%5BJournal%5D)">Age-related performance fatigability: a comprehensive review of dynamic tasks.</a> J Appl Physiol. 133 (4), 850-866 (2022).</li>
<li>Hinks, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Time+course+changes+in+in+vivo+muscle+mechanical+function+and+Ca2%2B+regulation+of+force+following+experimentally+induced+gradual+ovarian+failure+in+mice%5BTitle%5D)+AND+%22Exp+Physiol%22%5BJournal%5D)">Time course changes in in vivo muscle mechanical function and Ca2+ regulation of force following experimentally induced gradual ovarian failure in mice.</a> Exp Physiol. 109 (5), 711-728 (2024).</li>
<li>Edman, K. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Double-hyperbolic+force-velocity+relation+in+frog+muscle+fibres%5BTitle%5D)+AND+%22J+Physiol%22%5BJournal%5D)">Double-hyperbolic force-velocity relation in frog muscle fibres.</a> J Physiol. 404 (1), 301-321 (1988).</li>
</ol>

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sabit yük (İzotonik) ve sabit hız (İzokinetik) tork-hız-güç profillerinin oluşturulması sıçan plantar fleksörleri için İn vivo

Construction of Constant-Load (Isotonic) and Constant-Velocity (Isokinetic) Torque-Velocity-Power Profiles In vivo for the Rat Plantar Flexors

Sabit Yük (İzotonik) ve Sabit Hız (İzokinetik) Tork-Hız-Güç Profillerinin Oluşturulması Sıçan Plantar Fleksörleri için İn vivo

Understanding the torque-velocity-power relationship of muscle is critical for assessing dynamic muscle performance, which has implications for health and ...

construction-constant-load-isotonic-constant-velocity-isokinetic

Research

JoVE Journal

Bioengineering

Biyomühendislik

Biz köpek hindlimb kas gücü ve tekrarlanan eksantrik kasılmalar kas yanıtı ölçmek için bir minimal invaziv ve ağrısız bir yöntem açıklanmaktadır.

Vivo Köpek Kas Fonksiyon Testi

Bir In vivo</emDaralma indüklenen Sakatlık ve Kurtarma Non-invaziv İzleme> Kemirgen Model

Bu video cerrahi hazırlık ve sıçan medial gastroknemius kas hazırlık kasılma cevapları incelemek için gerekli prosedürleri...

Rat Prosedürleri<em&gt; In situ</em&gt; İskelet Kası Kontraktil Özellikleri

Nöromüsküler fonksiyondaki değişiklikleri değerlendirmek için bir protokol sunuyoruz. Perkütan elektriksel sinir stimülasyonu, kas yanıtlarını uyandıran non-invaziv bir yöntemdir. Bu yanıtların elektrofizyolojik ve mekanik özellikleri, beyinden kasa kadar nöromüsküler fonksiyonun değerlendirilmesine izin verir (supra-spinal, spinal ve periferik seviyeler).

Perkütan Elektriksel Sinir Stimülasyonu kullanma Nöromüsküler Fonksiyonu Değerlendirilmesi

Peroneal sinirin uyarılmasını ve sıçan arka ayağının anterior krural kompartmanının kasılmasını takiben ayağın dorsifleksiyonunu ölçmek için bir in vivo protokol tarif ediyoruz. Bu tür ölçümler, iskelet kası patolojisini ve kas onarımı ve rejenerasyonuna yönelik doku mühendisliği yaklaşımlarını değerlendirmek için vazgeçilmez bir translasyonel araçtır.

Uygulamaları<em&gt; İn Vivo</em&gt; Doku değerlendirilmesi için Rat Tibialis Anterior Fonksiyonel Test İskelet Kası Onarım Engineered

Motor geri kazanımın değerlendirilmesi deneysel periferik sinir çalışmalarında kıyaslama sonucu ölçüsü olmaya devam etmektedir. Sıçandaki tibialis ön kasının izometrik tetanik kuvvet ölçümü, siyatik sinir kusurlarının yeniden yapılandırılmasından sonra fonksiyonel sonuçları değerlendirmek için paha biçilmez bir araçtır. Yöntemler ve nüanslar bu makalede ayrıntılı olarak açıklanmıştır.

Sıçandaki Tibialis Ön Kasının Maksimum İzometrik Tetanik Kuvvet Ölçümü

Diz ekstensor maksimal gücünün ölçülmesi yaşlanma, hastalık, yaralanma ve rehabilitasyona fonksiyonel adaptasyonları anlamak için zorunludur. In vivo diz uzatma izometrik tepe tetanik tork tekrar tekrar ölçmek için yeni bir yöntem sunuyoruz.

Farelerde Diz Ekstensör Kas fonksiyonunun in vivo ölçümü

Bu protokol, anestezi uygulanan domuzlarda ortak peroneal sinirin elektriksel stimülasyonu yoluyla elde edilen in vivo tork verilerinin değerlendirilmesi ve yorumlanması ile ilgili kısa deneysel ayrıntıları açıklamaktadır.

İn Vivo Domuzdan Hindlimb Dorsiflexor İzometrik Tork Ölçümü

Bu makale, gerinim geçişlerinin ve aktivasyonun kas kuvveti tepkisine katkılarını değerlendirmek için bir laboratuvar kemirgeninden bir "avatar" kası kullanılarak ex vivo iş döngüsü deneyleri sırasında in vivo kas kuvveti üretimini taklit etme metodolojisini detaylandırır.

"Avatar", In vivo Gerinim ve Aktivasyon Kullanılarak Modifiye Edilmiş Bir Ex vivo Çalışma Döngüsü Deneyi

Bu protokol, aşırı kullanıma bağlı tendinopatinin in vivo bir modeli için bir sıçan Aşil tendonunda ölçülebilir ve kontrollü yorgunluk yaralanmalarını indüklemek için kullanılan bir test sistemi sunar. Prosedür, sıçanın ayak bileğini, özel olarak yazılmış bir MATLAB komut dosyası ile pasif ayak bileği dorsifleksiyonu gerçekleştiren bir eklem aktüatörüne sabitlemekten oluşur.

Aşırı Kullanıma Bağlı Tendinopatinin In Vivo Modeli için Pasif Ayak Bileği Dorsifleksiyon Test Sistemi

Öğrenin sıçan plantar fleksörlerinde izotonik ve izokinetik tork-hız-güç profillerini in vivo ölçme yöntemini. Bu protokol kas mekaniğini ve hastalıkların kas fonksiyonuna etkisini anlamak için kullanılır.

Sabit Yük (İzotonik) ve Sabit Hız (İzokinetik) Tork-Hız-Güç Profillerinin Oluşturulması Sıçan Plantar Fleksörleri için İn vivo

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