Name: Kendi Kendini Oluşturan İnsan Kardioidlerinde Hipoksi-Reoksijenlenme Yaralanma Modeli (Video) | JoVE Journal
Uploaded: 2026-03-17T12:35:00.000+00:00
Duration: 10 min 41 s
Description: Keşfedin iPSC kaynaklı insan kardioidlerinde hipoksi-reoksijenlenme yaralanma modelini. Bu yöntem, iskemi/reperfüzyon hasarını taklit ederek kardiyak rejenerasyon tedavilerini test etmeye olanak tanır.

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</ol>

<table><tbody><tr><td>Accutase çözeltisi</td><td>Sigma-Aldrich</td><td>A6964-500ML</td><td></td></tr><tr><td>Anti-Kardiyak Troponin T antikoru</td><td>Abcam</td><td>EPR20266</td><td></td></tr><tr><td>B-27 Takviyesi (50X), serumsuz</td><td>Gibco</td><td>17504044</td><td></td></tr><tr><td>B-27 Takviyesi, insülin hariç</td><td>Gibco</td><td>A1895602</td><td></td></tr><tr><td>BioTek Synergy Neo2 Plaka Okuyucu</td><td>BioTek</td><td></td><td></td></tr><tr><td>Cal-520,</td><td>AAT Bioquest</td><td>21131</td><td></td></tr><tr><td>CardioCount V1</td><td>Karra Laboratuvarı</td><td>PMID: 38984052</td><td></td></tr><tr><td>Hücre hattı (Homo sapiens, erkek) - DU11 iPSC Hattı</td><td>Duke Üniversitesi Kök Hücre Çekirdeği</td><td></td><td></td></tr><tr><td>Şarjlı Mikroskop Slaytları, Beyaz Cam</td><td>VWR</td><td>89500-496</td><td></td></tr><tr><td>CHIR 99021</td><td>Tocris</td><td>4423</td><td></td></tr><tr><td>CloneR</td><td>STEMCELL Teknolojileri</td><td>5888</td><td></td></tr><tr><td>Corning 96-Hole Şeffaf Ultra Düşük Bağlantı Mikroplakaları</td><td>Fisher</td><td>7007</td><td></td></tr><tr><td>Tek Kullanımlık Pipetleme Rezervuarları</td><td>VWR</td><td>89094-678</td><td></td></tr><tr><td>DMEM/F-12, HEPES</td><td>Gibco</td><td>11330057</td><td></td></tr><tr><td>EVOS Floresan Mikroskop</td><td>EVOS</td><td></td><td></td></tr><tr><td>Geltrex Azaltılmış Büyüme Faktörü Bazal-Membran Matrisi, LDEV içermemiş, kök hücre nitelikli</td><td>Gibco</td><td>A1413302</td><td></td></tr><tr><td>İnsan Aktivin Bir Rekombinant Protein</td><td>PEPROTECH INC/Life Technologies</td><td>120-14E-50UG</td><td></td></tr><tr><td>IWP-2</td><td>STEMCELL Teknolojileri</td><td>72124</td><td></td></tr><tr><td>L-Askorbin Asit (Kristal/Sertifikalı ACS)</td><td>Fisher Scientific</td><td>A61-100</td><td></td></tr><tr><td>Fare anti-Vimentin</td><td>DSHB</td><td>AMF-17b</td><td></td></tr><tr><td>mTeSR Plus</td><td>STEMCELL Teknolojileri</td><td>100-0276</td><td></td></tr><tr><td>Multiwell Hücre Kültür Plakaları &ndash; 12 iyi</td><td>VWR</td><td>10062-894</td><td></td></tr><tr><td>Plüronik F-127</td><td>Sigma-Aldrich</td><td>P2443</td><td></td></tr><tr><td>ReLeSR</td><td>STEMCELL Teknolojileri</td><td>100-0483</td><td></td></tr><tr><td>RPMI 1640 Orta</td><td>Gibco</td><td>11875119</td><td></td></tr><tr><td>RPMI 1640 Orta, fenol kırmızısı yok</td><td>Gibco</td><td>11835-030</td><td></td></tr><tr><td>Thiazovivin</td><td>STEMCELL Teknolojileri</td><td>72254</td><td></td></tr><tr><td>Tissue-Tek O.C.T. Bileşimi</td><td>SAKURA FINETEK USA INC (VWR)</td><td>25608-930</td><td></td></tr><tr><td>Zeiss Dönen Disk Konfokal Mikroskobu</td><td>Zeiss</td><td></td><td></td></tr></tbody></table>

kendi kendini oluşturan İnsan kardioidlerinde hipoksi-reoksijenlenme yaralanma modeli

A Hypoxia-Reoxygenation Injury Model in Self-Assembling Human Cardioids

Kendi Kendini Oluşturan İnsan Kardioidlerinde Hipoksi-Reoksijenlenme Yaralanma Modeli

Without rapid revascularization, ischemic heart disease leads to irreversible loss of cardiomyocytes, scar formation, and decreased cardiac function. The ...

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Medicine

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Bu el yazması, yüzey işaretleyici ekspresyonu ve hücre sıralaması kullanılarak tanımlanmış tasarlanmış kardiyak dokuların oluşturulmasını açıklar. Tanımlanan dokular daha sonra insan kalbinin işlevsel, ancak kontrollü bir model sistemini sağlamak için kardiyak hücre tedavisi mekanizmalarını araştırmak için çok dokulu bir biyoreaktörde kullanılabilir.

Tanımlı İnsan Engineered Kalp Dokuların inşaatı Kardiyak Hücre Tedavisinin Mekanizmaları Eğitim için

Burada, kan nakli ya da bir fare inotropic ajanlar olmadan basit kurtarma Kardiyopulmoner bypass modeli tanımlamak için bir iletişim kuralı mevcut. Bu model birden fazla organ sekel Kardiyopulmoner baypas uzun vadeli bir çalışma sağlar.

Kan nakli veya Sıçanlarda Inotropic ajanlar olmadan kurtarma Kardiyopulmoner Bypass modeli

İskemiye neden olan doku hasarının kantitatif değerlendirilmesi için bir yöntem le birlikte, insan kaynaklı pluripotent kök hücrelerden elde edilen kardiyomiyositler kullanılarak iskemik kalp hastalığı nın bir modelini saklı atıyoruz. Bu model ilaç tarama ve iskemik kalp hastalığı üzerinde daha fazla araştırma için yararlı bir platform sağlayabilir.

HiPSC Türetilmiş Kardiyomiyosit Kullanarak İskemik Kalp Hastalığı Modeli ve Kardiyomiyosit Kontraksiyonunun Video Tabanlı Karşılaştırılması

Farklı hipotermi derecelerinin miyokard koruması üzerindeki belirgin etkileri ayrıntılı olarak değerlendirilmemiştir. Bu çalışmanın amacı, insan kardiyomiyosit tabanlı bir modelde farklı hipotermi tedavilerinin ardından hücre ölüm seviyelerini ölçmek ve gelecekteki derinlemesine moleküler araştırmaların temelini atmaktı.

İnsan Kardiyak Miyosit Modelinde Hipotermi-Önkoşullama Sonrası Miyokard Korumasının İn vitro Değerlendirmesi

Bu protokolün amacı, kardiyak doku mühendisliği, ilaç taraması ve hastalık modelleme uygulamaları için biyomimetik, kollajen bazlı bir hidrojel içinde kardiyak fibroblastlar (KF' ler) ile birlikte kültürlenmiş kök hücre türevi kardiyomiyositlerden oluşan, yüksek hizalanmış insan kardiyak dokusunun üç boyutlu (3D) mikroakışkan modelinin gelişimini açıklamak ve göstermektir.

Mikroakışkan Bir Platformda 3D Organize İnsan Kardiyak Dokusu Geliştirme

Uzamsal mesafe, ayrı endotel ve kardiyomiyosit hücre katmanlarının bir ko-kültür modelinde hipoksi / reoksijenasyon hasarının değerlendirilmesinde anahtar bir parametredir, bu da ilk kez, kardiyomiyosit korumasında endotel hücrelerinin rolünü test etmek için uygun bir in vitro model sağlamak için ko-kültür uzamsal ortamının optimize edilmesinin gerekli olduğunu düşündürmektedir.

Kardiyak İskemi / Reperfüzyonu In Vitro Taklit Etmek için Bir Hücre Ko-Kültür Modelinin Geliştirilmesi

Burada, insan pluripotent kök hücrelerini kendi kendine örgütlenerek verimli bir şekilde kullanarak gelişimsel olarak ilgili insan kalbi organoidleri (hSO'lar) oluşturmak için bir protokol açıklıyoruz. Protokol, gelişimsel ipuçlarının sıralı aktivasyonuna dayanır ve son derece karmaşık, işlevsel olarak ilgili insan kalp dokuları üretir.

Pluripotent Kök Hücrelerden Elde Edilen Kendi Kendine Monte Edilen İnsan Kalbi Organoidlerinin Üretilmesi

Kök hücre kaynaklı kardiyomiyositler kullanılarak biyomühendislik yapılan üç boyutlu kalp dokuları, doğal kardiyak nişin temel yönlerini özetlerken, sağlıklı ve hastalıklı insan miyokardını in vitro incelemek için umut verici modeller olarak ortaya çıkmıştır. Bu makale, insan kaynaklı pluripotent kök hücre kaynaklı kardiyomiyositlerden üretilen yüksek içerikli tasarlanmış kardiyak dokuların üretilmesi ve analiz edilmesi için bir protokolü açıklamaktadır.

Tasarlanmış Kardiyak Dokuların Veri Toplamasını ve Model Verimini İyileştirmek için Bir Biyoreaktör Tasarlama

Burada, yüksek metabolik proksimal tübüler epitel hücrelerinde hipoksi/reoksijenasyon hasarını modellemek için in vitro bir protokolü tanımlıyoruz. Bu model, proksimal tübul hasar belirteçlerinin ekspresyonu ve mitokondriyal solunum fonksiyonunun değerlendirilmesi yoluyla ölçülebilen metabolik stres kaynaklı hasarı indüklemek için tasarlanmıştır.

Proksimal tübüler epitel hücrelerinde hipoksi/reoksijenlenme yaralanmasının modellenmesi

Ciddi omurilik yaralanması bir kontüzyon modeli açıklanmıştır. Ayrıntılı ameliyat öncesi, ameliyat ve ameliyat sonrası adımları tutarlı bir model elde etmek açıklanmıştır.

Sıçanlarda Şiddetli Spinal Kord Yaralanması bir bere modeli

Keşfedin iPSC kaynaklı insan kardioidlerinde hipoksi-reoksijenlenme yaralanma modelini. Bu yöntem, iskemi/reperfüzyon hasarını taklit ederek kardiyak rejenerasyon tedavilerini test etmeye olanak tanır.

Kendi Kendini Oluşturan İnsan Kardioidlerinde Hipoksi-Reoksijenlenme Yaralanma Modeli

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