Name: Koroner Endarter Baypas Grefti Hastalarının Koroner Endarterektomi Dokusundan Tek Çekirdek İzolasyonu (Video) | JoVE Journal
Uploaded: 2026-04-03T13:08:00.000+00:00
Duration: 9 min 9 s
Description: Öğrenin, koroner endarterektomi dokusundan tek çekirdek izolasyonu yöntemini. Bu protokol, yüksek riskli aterosklerotik plakların transkriptomik profillemesini sağlar ve snRNA-seq için uygundur.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Roth, G. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Global+burden+of+cardiovascular+diseases+and+risk+factors-+1990-2019%5BTitle%5D)+AND+%22J.+Am.+Coll.+Cardiol%22%5BJournal%5D)">Global burden of cardiovascular diseases and risk factors- 1990-2019.</a> J. Am. Coll. Cardiol. 76 (25), 2982-3021 (2020).</li>
<li>de Winther, M. P. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Translational+opportunities+of+single-cell+biology+in+atherosclerosis%5BTitle%5D)+AND+%22Eur.+Heart+J%22%5BJournal%5D)">Translational opportunities of single-cell biology in atherosclerosis.</a> Eur. Heart J. 44 (14), 1216-1230 (2023).</li>
<li>Mohebbati, R., Momeni-Moghaddam, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+role+of+red+blood+cells+in+cholesterol+accumulation+and+atherosclerotic+plaque+instability%3A+a+perspective+on+atherosclerosis%5BTitle%5D)+AND+%22Curr.+Cardiol.+Rev%22%5BJournal%5D)">The role of red blood cells in cholesterol accumulation and atherosclerotic plaque instability: a perspective on atherosclerosis.</a> Curr. Cardiol. Rev. 21 (5), (2025).</li>
<li>Ajoolabady, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Inflammation+in+atherosclerosis%3A+pathophysiology+and+mechanisms%5BTitle%5D)+AND+%22Cell+Death+Dis%22%5BJournal%5D)">Inflammation in atherosclerosis: pathophysiology and mechanisms.</a> Cell Death Dis. 15 (11), 817(2024).</li>
<li>Dawson, L. P., Layland, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High-risk+coronary+plaque+features%3A+a+narrative+review%5BTitle%5D)+AND+%22Cardiol.+Ther%22%5BJournal%5D)">High-risk coronary plaque features: a narrative review.</a> Cardiol. Ther. 11 (3), 319-335 (2022).</li>
<li>Tong, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Highly+sensitive+magnetic+particle+imaging+of+vulnerable+atherosclerotic+plaque+with+active+myeloperoxidase-targeted+nanoparticles%5BTitle%5D)+AND+%22Theranostics%22%5BJournal%5D)">Highly sensitive magnetic particle imaging of vulnerable atherosclerotic plaque with active myeloperoxidase-targeted nanoparticles.</a> Theranostics. 12 (17), 7641-7641 (2022).</li>
<li>Lu, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Coronary+computed+tomography+angiography+assessment+of+high-risk+plaques+in+predicting+acute+coronary+syndrome%5BTitle%5D)+AND+%22Front.+Cardiovasc.+Med%22%5BJournal%5D)">Coronary computed tomography angiography assessment of high-risk plaques in predicting acute coronary syndrome.</a> Front. Cardiovasc. Med. 8, (2021).</li>
<li>Kinoshita, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High-risk+plaques+on+coronary+computed+tomography+angiography%5BTitle%5D)+AND+%22JACC+Cardiovasc.+Imaging%22%5BJournal%5D)">High-risk plaques on coronary computed tomography angiography.</a> JACC Cardiovasc. Imaging. 17 (4), 382-391 (2024).</li>
<li>Canu, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Non-invasive+multimodality+imaging+of+coronary+vulnerable+patient%5BTitle%5D)+AND+%22Front.+Cardiovasc.+Med%22%5BJournal%5D)">Non-invasive multimodality imaging of coronary vulnerable patient.</a> Front. Cardiovasc. Med. 9, (2022).</li>
<li>Liu, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Assessment+of+coronary+computed+tomography+angiography-derived+plaque+features+in+the+diagnosis+of+optical+coherence+tomography-defined+vulnerable+plaques%5BTitle%5D)+AND+%22Quant.+Imaging+Med.+Surg%22%5BJournal%5D)">Assessment of coronary computed tomography angiography-derived plaque features in the diagnosis of optical coherence tomography-defined vulnerable plaques.</a> Quant. Imaging Med. Surg. 15 (3), 2029-2041 (2025).</li>
<li>Chiorescu, R. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Vulnerable+atherosclerotic+plaque%3A+is+there+a+molecular+signature%5BTitle%5D)+AND+%22Int.+J.+Mol.+Sci%22%5BJournal%5D)">Vulnerable atherosclerotic plaque: is there a molecular signature.</a> Int. J. Mol. Sci. 23 (21), 13638(2022).</li>
<li>The applications of single-cell RNA sequencing in atherosclerotic disease. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=%22Front.+Cardiovasc.+Med%22">Front. Cardiovasc. Med</a>. Slenders, L., Tessels, D. E., van der Laan, S. W., Pasterkamp, G., Mokry, M. 9, (2022).</li>
<li>Adam, C. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Novel+biomarkers+of+atherosclerotic+vascular+disease-latest+insights+in+the+research+field%5BTitle%5D)+AND+%22Int.+J.+Mol.+Sci%22%5BJournal%5D)">Novel biomarkers of atherosclerotic vascular disease-latest insights in the research field.</a> Int. J. Mol. Sci. 23 (9), 4998(2022).</li>
<li>Ye, W., Chen, Z., Xia, Z., Li, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+RNA+sequencing+in+human+atherosclerotic+plaques+reveals+a+novel+smooth+muscle+cell+subtype+that+possesses+multi+differentiation+potential+and+shapes+the+microenvironment%5BTitle%5D)+AND+%22Clin.+Exp.+Med%22%5BJournal%5D)">Single-cell RNA sequencing in human atherosclerotic plaques reveals a novel smooth muscle cell subtype that possesses multi differentiation potential and shapes the microenvironment.</a> Clin. Exp. Med. 25 (1), 251(2025).</li>
<li>Kelly, J. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Coronary+endarterectomy%3A+analysis+of+the+society+of+thoracic+surgeons+adult+cardiac+surgery+database%5BTitle%5D)+AND+%22Ann.+Thorac.+Surg%22%5BJournal%5D)">Coronary endarterectomy: analysis of the society of thoracic surgeons adult cardiac surgery database.</a> Ann. Thorac. Surg. 114 (3), 667-674 (2022).</li>
<li>Ghatanatti, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Coronary+endarterectomy%3A+recent+trends%5BTitle%5D)+AND+%22J.+Clin.+DIAGNOSTIC+Res%22%5BJournal%5D)">Coronary endarterectomy: recent trends.</a> J. Clin. DIAGNOSTIC Res. , (2017).</li>
<li>Li, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+transcriptome+analysis+reveals+dynamic+cell+populations+and+differential+gene+expression+patterns+in+control+and+aneurysmal+human+aortic+tissue%5BTitle%5D)+AND+%22Circulation%22%5BJournal%5D)">Single-cell transcriptome analysis reveals dynamic cell populations and differential gene expression patterns in control and aneurysmal human aortic tissue.</a> Circulation. 142 (14), 1374-1388 (2020).</li>
<li>Alsaigh, T., Evans, D., Frankel, D., Torkamani, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Decoding+the+transcriptome+of+calcified+atherosclerotic+plaque+at+single-cell+resolution%5BTitle%5D)+AND+%22Commun.+Biol%22%5BJournal%5D)">Decoding the transcriptome of calcified atherosclerotic plaque at single-cell resolution.</a> Commun. Biol. 5 (1), 1084(2022).</li>
<li>Iqbal, F., Lupieri, A., Aikawa, M., Aikawa, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Harnessing+single-cell+RNA+sequencing+to+better+understand+how+diseased+cells+behave+the+way+they+do+in+cardiovascular+disease%5BTitle%5D)+AND+%22Arterioscler.+Thromb.+Vasc.+Biol%22%5BJournal%5D)">Harnessing single-cell RNA sequencing to better understand how diseased cells behave the way they do in cardiovascular disease.</a> Arterioscler. Thromb. Vasc. Biol. 41 (2), 585-600 (2021).</li>
<li>Ahmad, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single+cell+RNA+sequencing+of+haematopoietic+cells+in+fresh+and+frozen+human+atheroma+tissue%5BTitle%5D)+AND+%22Cardiovasc.+Res%22%5BJournal%5D)">Single cell RNA sequencing of haematopoietic cells in fresh and frozen human atheroma tissue.</a> Cardiovasc. Res. 121 (3), 396-404 (2025).</li>
<li>Depuydt, M. A. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microanatomy+of+the+human+atherosclerotic+plaque+by+single-cell+transcriptomics%5BTitle%5D)+AND+%22Circ.+Res%22%5BJournal%5D)">Microanatomy of the human atherosclerotic plaque by single-cell transcriptomics.</a> Circ. Res. 127 (11), 1437-1455 (2020).</li>
<li>Williams, J. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single+cell+RNA+sequencing+in+atherosclerosis+research%5BTitle%5D)+AND+%22Circ.+Res%22%5BJournal%5D)">Single cell RNA sequencing in atherosclerosis research.</a> Circ. Res. 126 (9), 1112-1126 (2020).</li>
<li>Kim, N., Kang, H., Jo, A., Yoo, S. -A., Lee, H. -O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Perspectives+on+single-nucleus+RNA+sequencing+in+different+cell+types+and+tissues%5BTitle%5D)+AND+%22J.+Pathol.+Transl.+Med%22%5BJournal%5D)">Perspectives on single-nucleus RNA sequencing in different cell types and tissues.</a> J. Pathol. Transl. Med. 57 (1), 52-59 (2023).</li>
<li>Gruenbaum, Y., Foisner, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Lamins%3A+Nuclear+intermediate+filament+proteins+with+fundamental+functions+in+nuclear+mechanics+and+genome+regulation%5BTitle%5D)+AND+%22Annu.+Rev.+Biochem%22%5BJournal%5D)">Lamins: Nuclear intermediate filament proteins with fundamental functions in nuclear mechanics and genome regulation.</a> Annu. Rev. Biochem. 84 (1), 131-164 (2015).</li>
<li>Zwerger, M., Ho, C. Y., Lammerding, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nuclear+mechanics+in+disease%5BTitle%5D)+AND+%22Annu.+Rev.+Biomed.+Eng%22%5BJournal%5D)">Nuclear mechanics in disease.</a> Annu. Rev. Biomed. Eng. 13 (1), 397-428 (2011).</li>
<li>Dechat, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nuclear+lamins%3A+major+factors+in+the+structural+organization+and+function+of+the+nucleus+and+chromatin%5BTitle%5D)+AND+%22Genes+Dev%22%5BJournal%5D)">Nuclear lamins: major factors in the structural organization and function of the nucleus and chromatin.</a> Genes Dev. 22 (7), 832-853 (2008).</li>
<li>Oh, J. -M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparison+of+cell+type+distribution+between+single-cell+and+single-nucleus+RNA+sequencing%3A+enrichment+of+adherent+cell+types+in+single-nucleus+RNA+sequencing%5BTitle%5D)+AND+%22Exp.+Mol.+Med%22%5BJournal%5D)">Comparison of cell type distribution between single-cell and single-nucleus RNA sequencing: enrichment of adherent cell types in single-nucleus RNA sequencing.</a> Exp. Mol. Med. 54 (12), 2128-2134 (2022).</li>
<li>Nott, A., Schlachetzki, J. C. M., Fixsen, B. R., Glass, C. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nuclei+isolation+of+multiple+brain+cell+types+for+omics+interrogation%5BTitle%5D)+AND+%22Nat.+Protoc%22%5BJournal%5D)">Nuclei isolation of multiple brain cell types for omics interrogation.</a> Nat. Protoc. 16 (3), 1629-1646 (2021).</li>
<li>Gulko, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Protocol+for+flow+cytometry-assisted+single-nucleus+RNA+sequencing+of+human+and+mouse+adipose+tissue+with+sample+multiplexing%5BTitle%5D)+AND+%22STAR+Protoc%22%5BJournal%5D)">Protocol for flow cytometry-assisted single-nucleus RNA sequencing of human and mouse adipose tissue with sample multiplexing.</a> STAR Protoc. 5 (1), 102893(2024).</li>
<li>Fernandez, D. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+immune+landscape+of+human+atherosclerotic+plaques%5BTitle%5D)+AND+%22Nat.+Med%22%5BJournal%5D)">Single-cell immune landscape of human atherosclerotic plaques.</a> Nat. Med. 25 (10), 1576-1588 (2019).</li>
<li>Galvis, A. E., Fisher, H., Camerini, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((NP-40+fractionation+and+nucleic+acid+extraction+in+mammalian+cells%5BTitle%5D)+AND+%22BIO-PROTOCOL%22%5BJournal%5D)">NP-40 fractionation and nucleic acid extraction in mammalian cells.</a> BIO-PROTOCOL. 7 (20), (2017).</li>
<li>Wirka, R. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Atheroprotective+roles+of+smooth+muscle+cell+phenotypic+modulation+and+the+TCF21+disease+gene+as+revealed+by+single-cell+analysis%5BTitle%5D)+AND+%22Nat.+Med%22%5BJournal%5D)">Atheroprotective roles of smooth muscle cell phenotypic modulation and the TCF21 disease gene as revealed by single-cell analysis.</a> Nat. Med. 25 (8), 1280-1289 (2019).</li>
<li>Pan, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+genomics+reveals+a+novel+cell+state+during+smooth+muscle+cell+phenotypic+switching+and+potential+therapeutic+targets+for+atherosclerosis+in+mouse+and+human%5BTitle%5D)+AND+%22Circulation%22%5BJournal%5D)">Single-cell genomics reveals a novel cell state during smooth muscle cell phenotypic switching and potential therapeutic targets for atherosclerosis in mouse and human.</a> Circulation. 142 (21), 2060-2075 (2020).</li>
<li>Bakken, T. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-nucleus+and+single-cell+transcriptomes+compared+in+matched+cortical+cell+types%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Single-nucleus and single-cell transcriptomes compared in matched cortical cell types.</a> PLoS One. 13 (12), e0209648(2018).</li>
<li>Litviňuková, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Cells+of+the+adult+human+heart%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">Cells of the adult human heart.</a> Nature. 588 (7838), 466-472 (2020).</li>
<li>Hu, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-nucleus+transcriptomic+survey+of+cell+diversity+and+functional+maturation+in+postnatal+mammalian+hearts%5BTitle%5D)+AND+%22Genes+Dev%22%5BJournal%5D)">Single-nucleus transcriptomic survey of cell diversity and functional maturation in postnatal mammalian hearts.</a> Genes Dev. 32 (19-20), 1344-1357 (2018).</li>
<li>Rani, U., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Evaluation+of+use+of+RPMI+Medium+to+preserve+cell+morphology+for+pleural%2Fperitoneal+fluid+cytology%5BTitle%5D)+AND+%22J.+Cytol%22%5BJournal%5D)">Evaluation of use of RPMI Medium to preserve cell morphology for pleural/peritoneal fluid cytology.</a> J. Cytol. 39 (1), 26-29 (2022).</li>
<li>Machado, L., Relaix, F., Mourikis, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Stress+relief%3A+emerging+methods+to+mitigate+dissociation-induced+artefacts%5BTitle%5D)+AND+%22Trends+Cell+Biol%22%5BJournal%5D)">Stress relief: emerging methods to mitigate dissociation-induced artefacts.</a> Trends Cell Biol. 31 (11), 888-897 (2021).</li>
<li>Slyper, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+single-cell+and+single-nucleus+RNA-seq+toolbox+for+fresh+and+frozen+human+tumors%5BTitle%5D)+AND+%22Nat.+Med%22%5BJournal%5D)">A single-cell and single-nucleus RNA-seq toolbox for fresh and frozen human tumors.</a> Nat. Med. 26 (5), 792-802 (2020).</li>
<li>Krishnaswami, S. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Using+single+nuclei+for+RNA-seq+to+capture+the+transcriptome+of+postmortem+neurons%5BTitle%5D)+AND+%22Nat.+Protoc%22%5BJournal%5D)">Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons.</a> Nat. Protoc. 11 (3), 499-524 (2016).</li>
</ol>

<table><tbody><tr><td>100 &mu; M süzgeci</td><td>Miltenyi Biotech&nbsp;</td><td>130-098-463</td><td></td></tr><tr><td>30 &mu; M süzgeci</td><td>Miltenyi Biotech&nbsp;</td><td>130-098-458</td><td></td></tr><tr><td>Sığır Serum Albümini</td><td>HiMedia</td><td>TC546-25G</td><td></td></tr><tr><td>Santrifüj</td><td>MPW</td><td>NA</td><td>https://mpw.pl/en/offer/mpw-352r</td></tr><tr><td>Bileşik Mikroskop</td><td>Nikon</td><td>NA&nbsp;</td><td>Nikon Eclipse Ts2 ters mikroskobu (https://www.microscope.healthcare.nikon.com/en_AOM/products/inverted-microscopes/eclipse-ts2)</td></tr><tr><td>Konfokal Mikroskop&nbsp;</td><td>Nikon</td><td>NA</td><td>Nikon Ti2-E ters konfokal mikroskop (https://www.microscope.healthcare.nikon.com/en_AOM/products/inverted-microscopes/eclipse-ti2-series)</td></tr><tr><td>Kapak Slipleri</td><td>HiMedia</td><td>BG011C</td><td></td></tr><tr><td>CRYOCHIL Geniş Ağızlı Numune Şişesi</td><td>Tarsons</td><td>883191</td><td></td></tr><tr><td>DAPI</td><td>Sigma</td><td>D9542-5MG</td><td></td></tr><tr><td>DPBS</td><td>Gibco (Thermofischer)</td><td>10010-023</td><td></td></tr><tr><td>Santrifüj Tüpü (15 mL)</td><td>Tarsons</td><td>546021</td><td></td></tr><tr><td>Akış sitometresi&nbsp;</td><td>BD Biosciences</td><td>663029</td><td>BD FACSLyric (3L12C Enstrüman CEIVD)</td></tr><tr><td>Hemositometre</td><td>Axibio</td><td>CCB 100</td><td></td></tr><tr><td>Mikrosantrifüj Tüpü (1.5 mL)</td><td>Tarsons</td><td>500010C</td><td></td></tr><tr><td>Mikrosantrifüj Tüpü (5 mL)</td><td>Tarsons</td><td>500050</td><td></td></tr><tr><td>MgCl2</td><td>Sigma</td><td>M1028-100ML</td><td></td></tr><tr><td>Mikro havan</td><td>Tarsons</td><td>160020</td><td></td></tr><tr><td>NaCl</td><td>Sigma</td><td>S9888-500G</td><td></td></tr><tr><td>NP-40</td><td>HiMedia</td><td>MB143-100ML</td><td></td></tr><tr><td>Nukleazsız su</td><td>Gibco (Thermofischer)</td><td>W4502-1L</td><td></td></tr><tr><td>RNase inhibitörü</td><td>Sigma</td><td>333540201</td><td></td></tr><tr><td>RPMI 1640</td><td>Gibco (Thermofischer)</td><td>11875093</td><td></td></tr><tr><td>Slide&nbsp;</td><td>Riviera</td><td>72910135</td><td></td></tr><tr><td>Tris-HÇL</td><td>Sigma</td><td>T2194-100ML</td><td></td></tr><tr><td>Trypan blue&nbsp;</td><td>Sigma</td><td>302643-25G</td><td></td></tr></tbody></table>

koroner endarter baypas grefti hastalarının koroner endarterektomi dokusundan tek Çekirdek İzolasyonu

Single Nuclei Isolation from Coronary Endarterectomy Tissue of Coronary Artery Bypass Graft Patients

İnsan koroner endarterektomi dokusu, CABG ameliyatı geçiren hastalardan toplandı. Doku çekirdek izolyasyonu için tartıldı ve işlendi, ayrıca izole edilmiş çekirdekler akış sitometrisi ve konfokal mikroskopi ile doğrulandı (Şekil 1).
Trypan Blue çekirdek izolasyon kalitesi değerlendirmesi RPMI ortamında tutulan doku tartıldı ve 247,8 mg ölçüldü. Doku doğranmış, ardından nükleer izolasyon için mikro tahrik kullanılarak lizis tamponu içinde homoje...

Koroner Endarter Baypas Grefti Hastalarının Koroner Endarterektomi Dokusundan Tek Çekirdek İzolasyonu

Atherosclerosis is a chronic, progressive inflammatory condition characterized by the accumulation of plaque in the walls of arteries. During the ...

single-nuclei-isolation-from-coronary-endarterectomy-tissue-coronary

Research

JoVE Journal

Biology

Biyoloji

Kardiyak çekirdeklerin yoğunluğu sedimentasyon ile izole edilmiş ve akım sitometri kardiyomiyosit çekirdeklerinin belirlemek ve sıralamak için pericentriolar malzeme 1 (PCM-1) karşı antikorlar ile immunolabeled edilir.

Post-mortem Doku gelen kardiyomiyosit Çekirdeklerin İzolasyonu

Kalp hastalıklarının hücre temelinde Mevcut bilgi çok hayvan modelleri üzerinde yapılan çalışmalara dayanmaktadır. Burada açıklamak ve insan ventrikülün küçük cerrahi örneklerinden tek bir canlı kardiyomiyositlerin elde etmek için yeni bir yöntem doğrulamak. İnsan ventriküler miyositler elektrofizyolojik çalışmalar ve uyuşturucu testi için kullanılabilir.

Taze Cerrahi Örneklerden İzolasyon ve İnsan Ventriküler kardiyomiyositlerin Fonksiyonel Karakterizasyonu

Kalp kapakçığı endotel hücrelerini (VEC'ler) izole etme yeteneği, kapak gelişimi, bakımı ve hastalık mekanizmalarını anlamak için kritik öneme sahiptir.  Burada, VEC'lerin gelişimsel ve hastalık süreçlerine katkısını belirleyen çalışmalara izin verecek FACS kullanarak embriyonik ve yetişkin Tie2-GFP farelerinden VEC'lerin izolasyonunu açıklıyoruz.

Murin Vana Endotel Hücreleri İzolasyon

Bu protokolün amacı, koroner dolaşımdan fare primer vasküler düz kas hücrelerinin izolasyonu ve kültür teknikleri (VSMC) göstermektir. VSMC'Ier izole edildikten sonra, pek çok standart kültür teknikleri için kullanılabilir.

Murin Koroner Damar düz kas hücrelerinin izolasyonu

Bu protokol, fare koroner endotel hücrelerini görüntüleme veya moleküler biyolojik deneyler yapmak amacıyla izole etme yöntemimizi paylaşmak için hazırlanmıştır.

Fare Koroner Endotel Hücreleri İzolasyon

İnsan kalp dokusu miyokardiyal rejenerasyonu için uygun multipotent perivasküler öncü hücre popülasyonlarının barındırır. Perisitler ve CD34 + CD146 - - adventisya hücreleri, insan miyokard burada tarif edilen teknik, yerli kan damarları, örneğin CD146 + CD34 ile ilgili iki multipotent stromal hücre popülasyonlarının eş zamanlı izolasyonu ve saflaştırılması mümkün kılar.

İnsan Kardiyak Doku perivasküler Multipotent Öncü hücre popülasyonlarının izolasyonu

Tümör endotel hücrelerinin tümör microenvironment önemli belirleyicileri ve hastalığın seyrini vardır. Burada, saf ve uygun endotel hücre insan kolorektal karsinom ve uyuşturucu testi ve Patogenez araştırmalarda kullanılmak üzere normal kolon yalıtımı için bir protokol açıklanmıştır.

Yalıtım Normal kolon ve kolorektal Karsinom - gelişmiş bir iletişim kuralı üzerinden insan endotel hücrelerinin

Bu çalışmanın amacı, kardiyomiyositleri yetişkin kalpten tekrarlıca izole etmek ve DNA içeriğini ve nükleasyonu ölçmek için bir yöntem geliştirmektir.

İmmünsitokimya ve Ploidi Analizi için Sabit Kalplerden Kardiyomiyositlerin İzolasyon

Biz bir sindirim tampon ardışık doku sindirim yoluyla sıçan kalplerinden endokardiyal ve koroner endotel hücrelerinin izolasyon için bir protokol sunmak, tekrarlayan santrifüj döngüleri hücre toplama, ve anti-sıçan CD31 mikroboncuklar kullanarak hücre arıtma.

Endokardiyal ve Koroner Endotel Hücrelerinin Sıçan Kalbinin Ventriküler Serbest Duvarından İzolasyon

Burada, hücre çekirdeği hazırlığını tanımlayan bir protokol sunuyoruz. Kardiyak dokunun mikrodiseksiyonu ve enzimatik ayrışmasından sonra, progenitör hücreler donduruldu, ardından tek çekirdekli RNA dizilimi için kullanılan saf canlı hücrelerin izolasyonu ve yüksek verimli dizileme analizleri ile transpozaz erişilebilir kromatin için tek çekirdekli tahlil yapıldı.

Epigenom ve Tek Hücre Çözünürlüğünde Gen Ekspresyon Profillemesi için Fare Kardiyak Progenitör Hücrelerinden Çekirdek İzolasyonu

Bu çalışma, ilerleyici koroner arter darlığına bağlı kronik miyokard iskemisi olan ve enfarktüs olmaksızın bozulmuş kardiyak fonksiyon ile sonuçlanan cerrahi domuz modelini sunmaktadır. İskemiyi takiben, hayvanlara kök hücrelerden türetilen eksozom yüklü kollajen yamasının epikardiyal yerleşimi ile pompa dışı koroner arter baypas grefti uygulanır. Bu yardımcı tedavi miyokardiyal fonksiyonu ve iyileşmeyi iyileştirir.

Eksozom Yüklü Kollajen Yaması ve Off-pump Koroner Arter Baypas Grefti ile Tedavi Edilen Kronik Miyokard İskeminin Cerrahi Domuz Modeli

Öğrenin, koroner endarterektomi dokusundan tek çekirdek izolasyonu yöntemini. Bu protokol, yüksek riskli aterosklerotik plakların transkriptomik profillemesini sağlar ve snRNA-seq için uygundur.

Koroner Endarter Baypas Grefti Hastalarının Koroner Endarterektomi Dokusundan Tek Çekirdek İzolasyonu

Daha fazla video keşfet