Name: Escherichia coli hücre istilasının nicelendirilmesi ve görselleştirilmesi için amikacin koruma testi (Video) | JoVE Journal
Uploaded: 2026-07-03T12:40:00.000+00:00
Duration: 9 min 22 s
Description: Öğrenin, amikasin koruma testiyle Epitel hücre istilasını nicelendirmek ve görselleştirmek için kullanılan bu yöntemi. Çok ilaç dirençli patojenlerin istilasını değerlendirmek için güvenilir bir alternatif sunar.

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<ol>
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<table><tbody><tr><td>75 cm2 Tissue culture flask</td><td>Fisher Scientific&nbsp;</td><td>FB012937</td><td></td></tr><tr><td>Amikacin disulfate&nbsp;</td><td>Research Products International&nbsp;</td><td>A20540-25.0</td><td></td></tr><tr><td>Antibiotic-Antimycotic (100x)</td><td>Gibco</td><td>15240062</td><td></td></tr><tr><td>Anti-E.coli antibody</td><td>Abcam</td><td>ab137967</td><td></td></tr><tr><td>BioLite microwell plates - 6 well</td><td>Fisher Scientific&nbsp;</td><td>12-556-004</td><td></td></tr><tr><td>Bovine serum albumin</td><td>Sigma-Aldrich</td><td>A7906</td><td></td></tr><tr><td>Collagen I, Rat tail, 100 mg</td><td>Corning</td><td>354236</td><td></td></tr><tr><td>Countess cell counting chamber slide</td><td>Invitrogen</td><td>C10312</td><td></td></tr><tr><td>DMEM, high glucose</td><td>Gibco</td><td>11965118</td><td></td></tr><tr><td>Fetal bovine serum</td><td>Corning</td><td>35-011-CV</td><td></td></tr><tr><td>Galcial acetic acid</td><td>Sigma-Aldrich</td><td>A6283</td><td></td></tr><tr><td>Glycine ultrapure</td><td>USBiological</td><td>G8165</td><td></td></tr><tr><td>Goat anti-Rabbit IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488</td><td>Invitrogen</td><td>A-11008</td><td></td></tr><tr><td>Hoechst 33342, trihydrochloride trihydrate</td><td>Invitrogen</td><td>H3570</td><td></td></tr><tr><td>Liofilchem&nbsp;MTS amikacin [AK] 0.016-256 &mu;g/mL</td><td>Fisher Scientific&nbsp;</td><td>22-777-704</td><td>Amikacin MIC Test Strips</td></tr><tr><td>Paraformaldehyde solution, 4% in PBS</td><td>Fisher Scientific&nbsp;</td><td>J19943.K2</td><td></td></tr><tr><td>PBS, 1x</td><td>Corning</td><td>21-040-CV</td><td></td></tr><tr><td>Poly-L-lysine coated sterile German glass coverslips for cell culture</td><td>Neuvitro</td><td>NC1327375</td><td></td></tr><tr><td>ProLong gold antifade mountant&nbsp;</td><td>Invitrogen</td><td>P36934</td><td></td></tr><tr><td>Rhodamine-Phalloidin</td><td>Invitrogen</td><td>R415</td><td>Kırmızı-turuncu 540/565 nm</td></tr><tr><td>Surface treated sterile tissue culture plates</td><td>Fisher Scientific&nbsp;</td><td>FB012929</td><td>24-well plates</td></tr><tr><td>Triton X-100 (Electrophoresis)</td><td>Fisher Scientific&nbsp;</td><td>BP151-100</td><td></td></tr><tr><td>Trypan blue</td><td>Invitrogen</td><td>T10282</td><td></td></tr><tr><td>Trypsin-EDTA (0.25%), phenol red</td><td>Gibco</td><td>25200056</td><td></td></tr><tr><td>Tween-20</td><td>Fisher Scientific&nbsp;</td><td>BP337-100</td><td></td></tr></tbody></table>

escherichia coli hücre istilasının nicelendirilmesi ve görselleştirilmesi için amikacin koruma testi

Amikacin Protection Assay for Quantification and Visualization of Escherichia coli Cell Invasion

Escherichia coli hücre istilasının nicelendirilmesi ve görselleştirilmesi için amikacin koruma testi

Epithelial cell invasion is a key phenotype for many enteric pathogens, including Salmonella, Shigella, and Vibrio. An emerging invasive pathogen is ...

amikacin-protection-assay-for-quantification-visualization

Research

JoVE Journal

Immunology and Infection

İmmünoloji ve Enfeksiyon

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Bakteriyel Yapışma Memeli Epitel Hücreleri üzerine In Vitro Testi

Üç boyutlu hücre dışı matriks içine tümör hücresi invazyonu modelleri daha iyi...

Modelleme ve Görüntüleme 3-Boyutlu Toplu Hücre Invasion

Kaynak: Cayron, J. ve Lesterlin, C. Stres Tedavileri Altında Bakteri Büyümesinin Çok Ölçekli Analizi. J. Vis. Uzm. (2019)Bu video, canlılığı ölçmek için kaplama ve hücre boyutunu ve DNA içeriğini analiz etmek için akış sitometrisi kullanarak hücre bölünmesini inhibe eden antibiyotik tedavisi altında E. coli filamentasyonunu ve DNA replikasyonunu değerlendirme prosedürünü göstermektedir.

E. coli'de Antibiyotiğe Bağlı Filamentasyonun Kaplama Testi ve Akış Sitometrisi Kullanılarak Değerlendirilmesi

Bu videoda, hücre dışı matris sertliğine bağlı Listeria monocytogenes'in endotel hücreleri yoluyla yayılımını değerlendirmek için kantitatif hızlandırılmış mikroskopi gösterilmektedir. Daha yumuşak bir hidrojel, daha sert muadillerine kıyasla endotel hücreleri yoluyla daha hızlı bakteri yayılımını kolaylaştırır ve matris sertliğinin bakteri yayılımı üzerindeki etkisini vurgular.

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Vakuolar Kopma Tek Hücre ölçümleri Hücre içi patojenlerin neden

Bir fare modeli enterohemorrhagic bioluminescence etiketli bakteri kullanarak E. coli (EHEC) kolonizasyon için detaylı bir protokol sunulmuştur. Kollarındaki bu bakteriler tarafından bir non-invaziv görüntüleme sistemi içinde canlı hayvan vivo içinde tespiti geçerli anlayışımız EHEC kolonizasyon ilerletebilirsiniz.

Non-invaziv Vivo içinde Bioluminescence sistemi tarafından fare konak Enterohemorrhagic Escherichia Coli kolonizasyon algılama

Bakteriyel enfeksiyon yapisan hücre hücre dışı matriks sertlik etkisi problama için çok iyi biçim polyacrylamide tabanlı tahlil geliştirdik. Bu tahlil akış sitometresi, immunostaining ve çekiş kuvvet mikroskobu, hücreler, hücre dışı matriks ve patojen bakteriler arasında biyomekanik etkileşimlerin nicel ölçümler için izin ile uyumludur.

Hücre dışı matriks sertlik etkisi yapışık hücreleri bakteriyel enfeksiyon türleri çalışmak için çok iyi Format Polyacrylamide tabanlı tahlil

Geçerli protokol kanser hücre göç ve gerçek zamanlı olarak tek bir platformda işgali araştırma, kolayca tekrarlanabilir ve zamanı verimli bir seçenek hücre hareketlilik ve morfoloji çalışmaya sağlayan entegre bir yöntemini açıklar.

Otomatik bir canlı hücre Imager kullanarak basit geçiş/işgali iş akışı

Bu makalede, kokültürdeki bakteriyel rekabeti görselleştirmek ve ölçmek için tek hücreli floresan mikroskopisi kullanma yöntemi açıklanmaktadır.

Tek Hücreli Floresan Görüntüleme Kullanılarak Interbakteriyel Rekabetin Nicelemesi

Öğrenin, amikasin koruma testiyle Epitel hücre istilasını nicelendirmek ve görselleştirmek için kullanılan bu yöntemi. Çok ilaç dirençli patojenlerin istilasını değerlendirmek için güvenilir bir alternatif sunar.

Escherichia coli hücre istilasının nicelendirilmesi ve görselleştirilmesi için amikacin koruma testi

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