1. Equipment
2. Stimulus and Experiment Design

Figure 1. Sequence of events in the spatial cueing paradigm used to measure the consequences of visual attention. Each trial begins the same way, as shown in frame one, with a central fixation cross and two green boxes on either side. In frame two, the fixation cross is replaced by an arrow, pointing to one of the two boxes (50% of the time each). Finally, in frame three a letter is shown-either an L or a T-in one of the two boxes. In the example shown, the letter is an L. In the right panel example, the letter appears in the box that the arrow points to, producing a congruent trial. In the panel on the left, the letter appears opposite the arrow, producing an incongruent trial. The measure of interest is the time it takes a participant to make a correct response (the reaction time), in particular, the average difference between congruent and incongruent trials.

Figure 2. Sample table for organizing data output in a spatial cueing experiment. The primary measure of interest is the reaction time on each trial. In addition, the condition needs to be recorded in order to compare reaction time in congruent and incongruent trials, and the letter type and response given are necessary in order to evaluate response accuracy. It is also a good idea to record letter position to ensure that trials appear in the correct proportions. Please click here to view a larger version of this figure.
3. Running the Experiment
4. Analyzing the Results

Figure 3. A data table populated with results from 25 spatial cueing trials. The final column, labelled 'Accuracy,' was added after the experiment was completed, and a formula was used to automate an accuracy check. Please click here to view a larger version of this figure.
Source: Laboratory of Jonathan Flombaum—Johns Hopkins University
Attention refers to the limited human ability to select some information for processi…
1. Equipment
2. Stimulus and Experiment Design

Figure 1. Sequence of events in the spatial cueing paradigm used to measure the consequences of visual attention. Each trial begins the same way, as shown in frame one, with a central fixation cross and two green boxes on either side. In frame two, the fixation cross is replaced by an arrow, pointing to one of the two boxes (50% of the time each). Finally, in frame three a letter is shown-either an L or a T-in one of the two boxes. In the example shown, the letter is an L. In the right panel example, the letter appears in the box that the arrow points to, producing a congruent trial. In the panel on the left, the letter appears opposite the arrow, producing an incongruent trial. The measure of interest is the time it takes a participant to make a correct response (the reaction time), in particular, the average difference between congruent and incongruent trials.

Figure 2. Sample table for organizing data output in a spatial cueing experiment. The primary measure of interest is the reaction time on each trial. In addition, the condition needs to be recorded in order to compare reaction time in congruent and incongruent trials, and the letter type and response given are necessary in order to evaluate response accuracy. It is also a good idea to record letter position to ensure that trials appear in the correct proportions. Please click here to view a larger version of this figure.
3. Running the Experiment
4. Analyzing the Results

Figure 3. A data table populated with results from 25 spatial cueing trials. The final column, labelled 'Accuracy,' was added after the experiment was completed, and a formula was used to automate an accuracy check. Please click here to view a larger version of this figure.
Our ability to select certain information in an environment to process, while ignoring other stimuli, is referred to as attention.
Visual attention can either be overt?where the eyes are consciously aimed towards an object, like a rising full moon?or covert, in which a person notices something that they are not looking at directly.
For example, an individual might be staring at a sign pointing towards the left side of a fork in the road. However, they will still discern a nearby owl further down that path, because that?s the direction they are cued to go. This concept is referred to as spatial cueing?where covert attention is shifted by a particular signal.
Based on previous work by psychologist Michael Posner, this video demonstrates how to execute a computerized spatial cueing task, including how to interpret data investigating a measure of covert visual attention?reaction times across congruent and incongruent trials.
In this experiment, participants must detect and report brief visual targets that showcase focus and subsequent shifts in attention.
During every trial, participants are asked to observe three frames that occur in order: In frame 1, a red fixation cross, made of ?-in. long lines, is located in the center of the display. Two green boxes, each 1 by 1 in., are centered vertically, 1.5 in. away from the edges of the display.
After 100 ms, the second frame appears for this same duration, but this time, the fixation cross is replaced with a cue?a red arrow that points towards one of the two green boxes.
In the third frame, the cue arrow is simultaneously replaced with the fixation cross. In half of the trials, the letter 'T' is added to one of the two boxes, whereas the other half contains the letter 'L'; both are equally distributed. Participants are asked to identify the letter shown.
Following every response, a brief 500-ms inter-trial-interval occurs, and the sequence is repeated for a total of 400 trials.
Here, the trick is that they are either congruent, where the letter appears in the box that the arrow is pointing to 80% of the time, or incongruent, where it appears opposite of the arrow?s direction for 20% of the trials.
The dependent variable is then the time it takes a participant to make a correct response across trial types, which is achieved by simply choosing the letter shown in the box, regardless of the side.
Participants are expected, on average, to be faster at responding during congruent trials compared to incongruent ones, thus showing the advantages associated with cueing the spatial location of where one should focus their attention.
In preparation for the experiment, open the software program and verify that the spatial cueing paradigm is working correctly.
After recruiting participants, bring each one into the lab and explain that the task is designed to investigate the nature of visual attention. Before proceeding, ask them to complete an informed consent form.
To begin, seat the participant in front of the testing computer, with the back of their chair 60 cm away from the monitor. Explain the task instructions and answer any questions.
When the participant is ready, allow them to start the program by pressing the spacebar. Observe them over a few trials to ensure that they are either pressing the key 'L' or 'T' as soon as the letter appears on the screen.
Leave the testing room as they complete the 400 trials. Halfway through the experiment, provide a 2-min break, making the total task time less than 10 min.
To begin data analysis, first retrieve the captured data that were initially programmed into an output file.
Note that data for the following items should automatically be populated into the table: the trial number, the letter position, the letter type, the condition, the actual response given by the participant, and importantly, the reaction time?measured from the onset of the letter to the keypress.
Next, check whether the responses provided are accurate by adding a column called 'Accuracy' to the table. To populate this column, create a formula to compare 'Letter Type' with the 'Response Given', such that a 1 represents a correct response and 0 indicates an incorrect answer.
Now, verify that the total averaged accuracy values for each participant are above 0.8 to ensure that participants understood the task instructions.
To visualize the data, graph the average reaction times across participants by trial type. Note that they responded about 200 ms faster in congruent compared to incongruent trials.
This difference suggests that the arrow cued participants to attend to a particular spatial location, allowing them to more quickly process and identify the letter when it appeared there.
Now that you are familiar with designing an experiment to examine spatial cueing, let?s examine how researchers have used variations of the paradigm to investigate how attentional ability changes in cases of brain injury along with alterations in task demands.
Studies using functional magnetic resonance imaging indicated that regions within the parietal lobe are involved in the ability to orient attention to a spatial location.
In patients with focal damage due to strokes or tumors, Posner and colleagues discovered that reaction times were longer during incongruent compared to congruent trials and notably, when compared to neurological controls?those with lesions outside of the parietal area?which confirm the functional significance of this region.
Also, as you?ve learned already, the inclusion of cues in the task leads to anticipatory thoughts of where to focus attention, even though those expectations might not be met.
Researchers have adapted the paradigm to identify the kinds of stimuli, like unexpected bright flashes, that may automatically cause attention to shift. Such modifications could benefit individuals that may have trouble focusing under constrained demands, like those with Attention-Deficit-Hyperactivity Disorder.
You?ve just watched JoVE?s introduction to spatial cueing. Now you should have a good understanding of how to design and conduct a covert visual attention paradigm as well as how to analyze and interpret attentional demands when cues are both expected and mismatched.
Thanks for watching!
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Q1: What is the difference between overt and covert visual attention?
Overt attention involves consciously directing your eyes toward an object, like focusing on a rising moon. Covert attention occurs when you notice something without looking directly at it, such as detecting an owl down a path while staring at a sign. Spatial cueing demonstrates how covert attention shifts based on directional signals without eye movement.
Q2: How does the spatial cueing paradigm measure visual attention?
The spatial cueing paradigm uses reaction times as the primary measure of visual attention. Participants view a cue arrow pointing to one of two boxes, then identify a letter appearing in either box. Faster reaction times on congruent trials—when the letter appears where the arrow points—indicate that the cue successfully directed attention to that spatial location.
Q3: Why do participants respond faster on congruent trials compared to incongruent trials?
The arrow cue directs participants to anticipate where the letter will appear. On congruent trials, the letter appears in the expected location, allowing faster processing and identification. On incongruent trials, the letter appears opposite the cue direction, requiring attention to shift, which delays response time by approximately 200 milliseconds.
Q4: What brain regions are involved in orienting spatial attention?
Functional magnetic resonance imaging studies indicate that the parietal lobe is critical for orienting attention to spatial locations. Research by Posner and colleagues found that patients with focal parietal damage showed prolonged reaction times during incongruent trials compared to neurological controls with lesions outside this region, confirming the parietal lobe's functional significance.
Q5: How is accuracy verified in a spatial cueing experiment?
Researchers add an accuracy column to the data table and create a formula comparing the letter type shown with the participant's response, coding correct answers as 1 and incorrect answers as 0. Average accuracy values must exceed 0.8 across all trials to confirm participants understood task instructions and maintained focus throughout the experiment.
Q6: What modifications to the spatial cueing paradigm help study attention disorders?
Researchers have adapted the paradigm to identify stimuli like unexpected bright flashes that automatically capture attention. These modifications reveal how individuals with conditions like Attention-Deficit-Hyperactivity Disorder respond to competing attentional demands, helping identify which stimuli may benefit those struggling to focus under constrained task conditions.
Q7: How does Michael Posner's spotlight metaphor explain visual attention?
Posner likened attention to a spotlight that selectively illuminates portions of a scene. This metaphor captures how attention focuses on specific spatial locations while excluding other information. The spatial cueing paradigm operationalizes this concept by measuring how directional cues guide the spotlight of attention, demonstrating selective information processing in vision.
Chapters in this video
0:00
Overview
1:13
Stimulus and Experimental Design
3:17
Running the Experiment
4:23
Representative Results
5:56
Applications
7:25
Summary
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