Source: Laboratory of Jonathan Flombaum—Johns Hopkins University
Psychophysics is the name for a set of methods in perceptual psychology designed in o…
1. Stimuli and Equipment
2. Design

Figure 1. A flow chart for the design of an experiment using the auditory staircase procedure. The first trial always involves a tone played at an inaudible volume of 2 dB. Because the participant should not detect that tone, a 'No' response will be given, and the volume in the next trial will be increased by 1 dB (to 3 dB). Every trial (including and) following the second proceeds with the same directive: If a 'Yes' response is supplied by the participant, the volume in the next trial is reduced by 1 dB. And if a 'No' response is supplied, the volume in the next trial is increased by 1 dB. An experiment will include 30 trials per frequency.
3. Procedure
4. Analyzing the results

Figure 2. A sample of a table that includes the required outputs from an auditory staircase experiment. Note that data reported are for a single subject (labeled Subject #1) and for a single frequency (1000 Hz). The table includes three columns: the trial number, the volume of the tone presented on that trial (in dB) and the response given by the participant.

Figure 3. Sample results from a single participant and with a single tone. The graph plots the volume of the tone played, in dB, as a function of the trial number for each of the 30 trials. The main pattern is that the participant cannot hear any tone in the first few trials, producing a series of 'No' responses and prompting volume increases until the auditory threshold is reached. At that point, the participant moves back and forth between 'No' and 'Yes' responses allowing the researcher to identify the place at which sounds first become detectable.
One branch of perceptual psychology?psychophysics?is concerned with relating a stimulus?s actual compared to its perceived intensity.
Just like actual levels, perceptual ones can be measured: how bright a light must be for it to be observed, or how soft a sound can be for it to be heard.
For example, someone waiting for dinner to be served may not hear that it?s ready if they are at the base of the stairs; they have to climb a few steps before they hear anything, and maybe even a few more to Interpret the sounds.
This dynamic adjustment is the concept behind the staircase procedure, where the minimum noticed intensity can be reliably determined by stepping up or down the amount of stimulation.
This video demonstrates how to design and implement the staircase procedure, specifically to measure auditory thresholds?the minimal volume necessary for a tone to be perceived.
In this experiment, tones are presented through headphones at six different frequencies or pitches: 1?6 kHz?all within the human hearing range.
Given that our thresholds are not the same across all frequencies, six blocks are used to test each one independently. In each block, the frequency is briefly presented for 200 ms at volumes ranging from 2?40 dB.
The first tone is played at the lowest volume of 2 dB, a level that the participant should not perceive. If that?s the case, the volume on the next trial is increased by a step, 1 dB.
On the other hand, if it is noticeable, the volume is decreased by one. This procedure is repeated for 30 trials?resulting in staircase-like changes in volume.
The dependent variable is the participants? responses?whether they heard the tone or not. This information is then combined with the volume intensity data to determine the perceptual volume threshold at each frequency.
To begin the experiment, greet the participant in the lab and have them sit comfortably in front of the computer. Explain the task instructions: In each trial, the computer will play a tone through the headphones, after which you will be prompted to press the 'Y' key if you heard the tone or 'N' if you did not.
Allow the participant to put on the headphones, start the trials associated with the 1 kHz tone, and then leave the room.
After the first block of six frequencies is completed, return to the room and ask the participant to remove the headphones. Answer any questions they may have and give them a 2-min break.
When time is up, have the participant put the headphones back on to begin the trials related to the next tone. Repeat the steps until all six tones have been tested.
To analyze the results, generate a separate data table for each of the tones tested, with a column for trial number, volume level, and the participant?s responses.
During the first few trials, verify that they responded with a series of no?s, indicating that tones were inaudible at the start, which then should have prompted volume increases until the auditory threshold was reached.
Following verification, graph the volume played on each trial of every block as shown here for 1 kHz.
When the auditory threshold was reached, notice that the participant moved back and forth between 'No' and 'Yes' responses, which allows for the identification of what sounds first became detectable. The central tendency of this narrow range is a measure of the threshold.
To calculate the volume threshold at each tone, average the last 10 trials of every block and graph the results. Observe how this tended to increase as the frequency increased; in other words, low-pitched tones were easier to hear than high-pitched ones, which is due to the vibration properties of the filaments and bones of the ear.
Now that you are familiar with this efficient method for finding perceptual thresholds, let?s look at how it?s used to examine sensory decline in normal aging and with exposure to loud performances.
The staircase procedure has been used by researchers to examine how hearing thresholds change as humans age. In general, they found that volume thresholds increased as people get older. That is, for individuals aged 60, a high-frequency sound needed to be four times as loud as it would to be audible by those who are 20 years old.
Using similar methods, researchers also compared volume thresholds of people with normal hearing to those with impairments to identify the nature of the deficits. Specific frequencies were affected, such as at 4 and 5 kHz, whereas others were normal, suggesting that disease or damage is the cause, not aging.
In addition, the approach can be used to assess the consequences of various types of experiences on the auditory system. For example, studies have used a threshold approach to evaluate the effects of hearing loud heavy-metal music during a concert.
When researchers tested people just before attending a concert, and a half an hour after, they found that heavy metal increased the volume threshold for sounds. Thus, rock music can make you hard of hearing!
You?ve just watched JoVE?s video on the staircase procedure. Now you should have a good understanding of how to design a perceptual threshold task and run the experiment, as well as analyze and assess the results.
Thanks for watching!
View the full transcript and gain access to JoVE Science Education videos
Q1: What is psychophysics and why does it matter in perceptual psychology?
Psychophysics is a set of methods designed to relate the actual intensity of stimuli to their perceptual intensity. It addresses fundamental questions about human sensation: how bright must light be to detect it, or how soft can sound be and still be heard. Understanding these perceptual thresholds reveals the smallest amounts of stimulation humans can sense, which is essential for studying sensation and perception.
Q2: How does the staircase procedure work to find a perceptual threshold?
The staircase procedure is an efficient technique that systematically adjusts stimulus intensity based on participant responses. A tone starts at an inaudible level; if undetected, volume increases by 1 dB on the next trial. If detected, volume decreases by 1 dB. After 30 trials, this creates staircase-like fluctuations. The threshold is calculated by averaging the last 10 trials, identifying the narrow range where responses shift between 'no' and 'yes'.
Q3: Why are multiple frequencies tested separately in auditory threshold experiments?
Perceptual thresholds vary across different frequencies because of the vibration properties of the ear's filaments and bones. The staircase procedure tests six frequencies independently—from 1 to 6 kHz—to measure how volume thresholds change at each pitch. This reveals that low-pitched tones are generally easier to hear than high-pitched ones, providing a complete profile of auditory sensitivity.
Q4: What do the results reveal about hearing changes with age?
Researchers using the staircase procedure found that volume thresholds increase as people age. For individuals aged 60, high-frequency sounds needed to be four times as loud as they would for 20-year-olds to perceive them. This demonstrates age-related sensory decline in hearing, with older adults requiring greater stimulus intensity to detect sound across frequencies.
Q5: How can the staircase procedure distinguish between aging and hearing damage?
The staircase procedure identifies specific frequencies affected by disease or damage versus general aging effects. When comparing normal hearing to impaired hearing, researchers found that certain frequencies like 4 and 5 kHz showed elevated thresholds while others remained normal. This pattern suggests targeted damage rather than uniform age-related decline, helping clinicians diagnose the nature of hearing deficits.
Q6: What happens to hearing thresholds after exposure to loud music?
Studies using threshold measurement approaches found that exposure to loud heavy-metal music temporarily increases volume thresholds. When researchers tested participants before and 30 minutes after a concert, thresholds were elevated, meaning sounds needed to be louder to be detected. This demonstrates that intense acoustic exposure can cause temporary hearing sensitivity loss, illustrating how environmental factors affect auditory perception.
Q7: What data should be recorded and analyzed when running a staircase procedure experiment?
For each frequency tested, create a data table with trial number, volume level, and participant responses (yes or no). Graph volume changes across trials to visualize the staircase pattern. Calculate the threshold by averaging the last 10 trials of each block, then graph thresholds across all frequencies. This analysis reveals how detection sensitivity varies by pitch and identifies the minimal audible intensity at each frequency.