Using Diffusion Tensor Imaging in Traumatic Brain Injury

1. Participant recruitment

1. Recruit 20 participants with moderate to severe TBI, and 20 age-matched controls. All participants should be over the age of 18.
   1. TBI patients should have experienced a closed head injury that occurred at least 6 months ago. TBI is diagnosed by assessing several factors such as changes in consciousness, loss of consciousness, and loss of memory from before or after the accident. To be classified as moderate to severe, the patient must have experienced a period of loss of consciousness that was greater than 30 min, and/or obtained a score of less than 13 on the Glasgow Coma Scale.
   2. Control participants should have no history of neurological or psychological disorders.
   3. All participants should not have metal in their body. This is an important safety requirement due to the high magnetic field involved in MRI.
   4. All participants should not suffer from claustrophobia, since the MRI requires lying in the small space of the scanner bore.
2. Pre-scan procedures
   1. Fill out pre-scan paperwork.
   2. When participants come in for their MRI scan, have them first fill out a metal screen form to make sure they have no counter-indications for MRI, an incidental findings form giving consent for their scan to be looked at by a radiologist, and a consent form detailing the risks and benefits of the study.
   3. Prepare participants to go in the scanner by removing all metal from their body, including belts, wallets, phones, hair clips, coins, and all jewelry.
3. Scanner preparation
   1. Give the participant ear plugs to protect their ears from the noise of the scanner and ear phones to wear so they can hear the experimenter during the scan, and have them lie down on the bed with their head in the coil.
   2. Give the participant the emergency squeeze ball and instruct them to squeeze it in case of emergency during the scan.
   3. Use foam pads to secure the participants head in the coil to avoid excess movement during the scan, and remind the participant that it is very important to stay as still as possible during the scan, as even the smallest movements blur the images.
4. Data collection
   1. Collect a high-resolution T1-weighted anatomical scan. This will be used for registering the participant's brain to the standard atlas space.
   2. Begin scanning using a pulse sequence optimized for DTI.
      1. One B0 image is acquired that is not sensitive to diffusion direction.
      2. Multiple diffusion-weighted images are acquired, each sensitive to a different direction of diffusion. The more directions acquired, the more finely we will be able to resolve the diffusion tensor. However, increasing the number of directions also increases acquisition time. In this study, we will acquire 64 different directions.
5. Attention task
   1. Outside the MRI scanner, have all participants perform a version of the Attention Network Task (ANT)³ to assess their selective attention ability.
   2. Seat the participant in front of a computer screen, and instruct them on how to complete the task.
      1. Explain that a series of arrows will appear on the screen. The participant's task is to respond only to the arrow in the center, and to ignore the others. If the central arrow is pointing left, they will press the 'F' key with their left hand. If the central arrow is pointing right, they will press the 'J' key with their right hand. They should respond as quickly and as accurately as possible.
   3. Begin the task.
      1. On each trial, present a row of five arrows on the screen. Each arrow can be pointing left or right. On congruent trials, all of the arrows point in the same direction. On incongruent trials, the center arrow points in the opposite direction from the flanking arrows. Each trial begins with a fixation cross that remains on the screen for a variable duration between 400 and 1600 ms. Then the arrow stimuli appear and remain on the screen until the participant responds, or for a maximum of 1700 ms. The trial concludes with a fixation cross that remains on the screen until a total trial duration of 4s has been reached.
      2. Present 100 trials, half with congruent targets and half incongruent targets.
      3. Compute the difference in reaction time between incongruent targets and congruent targets. Typically, reaction time is slower in response to incongruent targets. People who are more distracted by the flanking arrows will have a larger difference in reaction time between incongruent and congruent targets. This measure of attentional control will be tested against measures of white matter integrity.
6. Post-experiment procedures
   1. Debrief the participant.
   2. Pay the participant.
7. Data analysis
   1. Pre-process the diffusion data.
      1. Visually inspect the data to make sure that it is free from artifacts.
      2. Perform eddy-current correction with specialized software.
      3. For each subject, register each of the directional diffusion images to the B0 image using a linear rigid-body affine transformation. This step will compensate for any motion that occurred from scan to scan.
      4. Remove the skull and other non-brain tissues from the images using automated software. This will ensure that we do not calculate tensors for voxels that are outside the brain.
      5. Combine among the multiple direction images to calculate the diffusion tensor at each voxel. There are several freely available software packages for processing DTI data that will calculate these values.
      6. Calculate FA at each voxel, the proportion of tensor magnitude due to anisotropic diffusion.
      7. Register the diffusion images to the high-resolution anatomical T1 image, and then to the standard atlas space to allow for group-level analysis.
   2. Define regions of interest (ROI).
      1. Obtain the three ROI masks from a standard white matter atlas. Here, we use the ICBM-DTI-81 white matter atlas created by the International Consortium for Brain Mapping (Figure 2).
      2. Register each individual subject's high-resolution anatomical image to the standard atlas.
      3. Warp the ROI masks into each participant's individual brain space using the registrations performed in the previous step.
   3. Extract FA values for each subject from each of the three ROIs.
   4. Compare the FA values between the two groups using Analysis of Variance (ANOVA).
   5. Compute the Pearson correlation between participants' congruency scores from the ANT and the FA values.