Robert M. Rioux and Taslima A. Zaman, Pennsylvania State University, Pennsylvania, PA
Users are responsible for the proper disposal of the waste gener…
1. Waste Management
2. Waste Collection and Storage
3. Sharps Disposal - Syringes and Needles
4. Glass Recycling
Hazardous waste, whether chemical, medical or radioactive, is generated in many laboratories and requires regulated disposal to ensure safety of public health and the environment.
The regulation of hazardous waste handling must be enforced from the moment of generation until its disposal at an offsite final destination facility.
Prior to commencing any laboratory activity, a waste management system must be devised. This is often established by an institute's Environmental Health and Safety, or EH&S, office, which enforces guidelines imposed by the Occupational Safety and Health Administration, or OSHA.
This video will illustrate the principles and typical laboratory procedures of proper waste disposal.
Efficient waste management is an important aspect of proper waste disposal. This can be accomplished by using the minimal amount of chemicals possible, by reusing surplus materials, and by recycling waste.
The generated waste must be labeled, segregated according to chemical compatibility, and stored in a fume hood or other well ventilated area. Other laboratory waste, such as sharps and glass, must be disposed with care in appropriate containers.
Now that we have discussed the principles of proper waste disposal, let's look at an actual procedure.
If applicable, keep chemical waste to a minimum by reducing the scale of operation. Furthermore, substitute chemicals with less hazardous reagents whenever possible.
In addition to minimizing the scale of operation, store only chemical quantities that will be used in the near term. You can also reduce chemical waste by recycling solvents like acetone, using a distillation.
Wear proper personal protective equipment including a lab coat, goggles, and gloves, as well as long pants and closed-toed shoes, whenever handling any chemical waste.
Collect chemical waste in suitable containers such as plastic carboys or glass bottles, and store near the point of generation in a designated satellite accumulation area.
Affix labels to the waste containers as soon as chemicals are added. Write on the labels the full names of the chemicals and their approximate compositions.
Additionally, use separate containers for halogenated, nonhalogenated, and aqueous waste to avoid potential heat or gas formation. When the containers are filled to capacity, carefully move them to a designated central accumulation area, from which they will be removed for disposal.
Dispose of chemically contaminated needles, syringes and razor blades, collectively known as sharps, inside of a sharps waste container.
For broken glass, used pipettes or test tubes, use a specialized glass waste container. If desired, empty bottles can be reused after triple rising with acetone, water, and again acetone.
You've just watched JoVE's introduction to proper waste disposal. You should now understand waste management, how to collect chemical and sharps waste, and how to store it for disposal. Thanks for watching!
View the full transcript and gain access to JoVE Science Education videos
Q1: Why is a waste management system necessary before starting laboratory work?
A waste management system must be established before commencing any laboratory activity to ensure safe handling of hazardous waste from generation through disposal. Your institute's Environmental Health and Safety office develops systems complying with regulations like OSHA standards to protect public health and the environment throughout the waste lifecycle.
Q2: What are the main strategies for reducing chemical waste in the laboratory?
Reduce chemical waste by minimizing operation scale, substituting hazardous reagents with less toxic alternatives, and storing only quantities needed for near-term use. You can also recycle solvents like acetone through distillation. These efficient waste management practices significantly decrease the volume of hazardous material requiring disposal.
Q3: How should chemical waste containers be labeled and organized?
Affix labels to waste containers immediately when chemicals are added, writing full chemical names and approximate compositions. Segregate waste into separate containers for halogenated, nonhalogenated, and aqueous materials to prevent dangerous heat or gas formation. Store containers in a designated satellite accumulation area near the point of generation.
Q4: What personal protective equipment is required when handling chemical waste?
Wear proper personal protective equipment including a lab coat, goggles, and gloves, along with long pants and closed-toed shoes whenever handling any chemical waste. This comprehensive protection safeguards against chemical exposure and contamination during waste collection, storage, and transport procedures.
Q5: How should sharps and broken glass be disposed of in the laboratory?
Dispose of chemically contaminated needles, syringes, and razor blades, collectively known as sharps, inside a designated sharps waste container. For broken glass, used pipettes, or test tubes, use a specialized glass waste container. Empty bottles can be reused after triple rinsing with acetone, water, and acetone again.
Q6: What happens to chemical waste after it reaches the central accumulation area?
Once containers are filled to capacity, they are carefully moved to a designated central accumulation area. From this central location, the waste is removed for disposal at an offsite final destination facility. Regulated handling continues throughout this transfer to ensure compliance with safety and environmental standards.
Q7: Where should chemical waste be stored before transport to the central accumulation area?
Store chemical waste in suitable containers such as plastic carboys or glass bottles in a designated satellite accumulation area near the point of generation. Containers must be kept in a fume hood or other well-ventilated area to minimize exposure and prevent vapor accumulation in the laboratory.