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Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos
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Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

DOI:
10.3791/1129-v

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12:21 min

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February 16, 2009

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, , , , ,
1Department of Molecular Cell and Developmental Biology,University of California, Los Angeles, 2Departments of Neurology and Neurobiology,University of California, Los Angeles

Chapters

  • 00:00Title
  • 00:47Introduction
  • 01:28Mounting Zebrafish Embryos for Long-Term Imaging
  • 03:25Two-photon axotomy using a custom built two-photon microscope with a Chameleon Ti-Sapphire laser
  • 04:42Two-photon axotomy on Zeiss 510 confocal/ two-photon microscope
  • 06:52Confocal time-lapse imaging on Zeiss LSM 510
  • 10:56Outcome and Results
  • 11:44Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a method for mounting zebrafish embryos for long-term imaging, two-photon imaging and tissue-damage techniques, and time-lapse confocal imaging.

Tags

Two-photon AxotomyTime-lapse Confocal ImagingZebrafish EmbryosCellular Mechanisms Of DevelopmentLong-term ImagingAutomated Image CaptureConfocal MicroscopePeripheral Sensory AxonsFemtosecond LaserTwo-photon MicroscopeGFP ExpressionSensory Neuron RegenerationAnesthetized EmbryosAgarose PositioningImaging Chamber

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