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Journal / Biology / Two-photon axotomy and time-lapse confocal…
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Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos
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Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

Article DOI: 10.3791/1129 • 12:21 min • February 16th, 2009
February 16th, 2009 •
1, 1, 1, 1, 2, 1
1Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, 2Departments of Neurology and Neurobiology, University of California, Los Angeles

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Summary
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Here we describe a method for mounting zebrafish embryos for long-term imaging, two-photon imaging and tissue-damage techniques, and time-lapse confocal imaging.

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Two-photon Axotomy Time-lapse Confocal Imaging Zebrafish Embryos Cellular Mechanisms Of Development Long-term Imaging Automated Image Capture Confocal Microscope Peripheral Sensory Axons Femtosecond Laser Two-photon Microscope GFP Expression Sensory Neuron Regeneration Anesthetized Embryos Agarose Positioning Imaging Chamber
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