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Optical Mapping of Langendorff-perfused Rat Hearts
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Optical Mapping of Langendorff-perfused Rat Hearts

Optical Mapping of Langendorff-perfused Rat Hearts

DOI:
10.3791/1138-v

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11:48 min

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August 11, 2009

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, ,
1Department of Anesthesiology, Perioperative and Pain Medicine,Children’s Hospital Boston and Harvard Medical School, 2Departments of Cardiac Surgery,Children’s Hospital Boston and Harvard Medical School

Chapters

  • 00:05Title
  • 00:11Introduction
  • 00:41Preparing the Solutions and the Langendorff System
  • 02:01Harvesting of the Rat Heart and Set-up of the Langendorff Perfusion System
  • 04:55Loading Potentiometric Dye and Acquiring Data
  • 08:45Analyze Acquisition Information Using RedShirt Imaging Software
  • 10:38Representative Visualization of a Myocardial Excitation Wave
  • 11:08Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a high temporal and spatial resolution technique to optically image action potential movement on the surface of Langendorff-perfused rat hearts using a potentiometric dye (di-8-ANEPPS).

Tags

Optical MappingLangendorff-perfused Rat HeartsVoltage-sensitive Fluorescent DyesElectrical ExcitationExperimental ModelsAction PotentialsArrhythmiasSpatiotemporal ResolutionsCustom-built EquipmentElectrophysiological MethodsComplementary Metal-oxide-semiconductor (CMOS) CamerasLight-emitting Diodes (LEDs)Voltage-sensitive DyesOpticsFiltersCardiovascular Cell BiologistsMicroscopesFluorescence Acquisition TechnologiesPhotodiode ArraysLaser Scanning SystemsCooled Charged-coupled Device (CCD) Cameras

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