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Measuring Caenorhabditis elegans Life Span on Solid Media

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Biology

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Measuring Caenorhabditis elegans Life Span on Solid Media

Measuring Caenorhabditis elegans Life Span on Solid Media

DOI:

•

12:49 min

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May 12, 2009

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George L. Sutphin², Matt Kaeberlein

¹Department of Pathology,University of Washington, ²Molecular and Cellular Biology Program,University of Washington

Chapters

00:00Title
01:06Introduction
01:28Prepare Nematode Growth Media (NGM) Plates
05:27Age-Synchronizing a Population of Animals
08:20Score Animals for Life Span
11:41Representative Results: Life Span Analysis
12:18Conclusion

Summary

Automatic Translation

In this article we present a general protocol for measuring life span of nematodes maintained on solid media with UV-killed bacterial food.

Tags

Caenorhabditis Elegans Life Span Measurement Solid Media Aging Cellular Deterioration Organelle Deterioration Mortality Model Organism Biology Of Aging Functional Decline Total Rate Of Aging Nematodes Responsiveness To External Stimuli Liquid Media Plates UV-killed Bacteria Diet Protocol Nematode Growth Media

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