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Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 2

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Biology

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JoVE Journal Biology

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 2

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 2

DOI:

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10:55 min

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April 10, 2009

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Judy Yen, Ron Golan, Kathleen Rubins

¹Whitehead Institute for Biomedical Research,MIT – Massachusetts Institute of Technology

Chapters

00:00Title
00:09Introduction
00:39cDNA synthesis from RNA
02:52Double-Stranded cDNA clean-up
05:55in vitro Transcription
06:31aRNA Clean-up After IVT
09:41Conclusion

Summary

Automatic Translation

Protocol for Vaccinia infection of HeLa cells and analysis of host and viral gene expression. Part 2 of 3.

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