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Chapters
- 00:04Introduction
- 00:18Preparation of solutions
- 00:54Assembling imaging system
- 02:26Perfusion
- 03:20Imaging
- 04:47Discussion
The ability to measure the kinetics of vesicle release can help provide insight into some of the basics of neurotransmission. Here we used real-time imaging of vesicles labeled with the red fluorescent dye FM 4-64 to measure the rate of presynaptic vesicle release in hippocampal neuronal cultures.
Tags
MeasuringExocytosisNeuronsFM LabelingKineticsVesicle ReleaseReal-time ImagingFM DyePresynapticEndocytosisFluorescenceSynaptic VesiclesLipophilic InteractionsBright SignalExtracellular FluidWash StepPlasma MembraneExpelledElectrical StimulationRate Of ReleaseFluorescence DecreaseNeuronal CulturesTransfected SynapsesControl Boutons
