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Biology

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A high-throughput method to globally study the organelle morphology in S. cerevisiae
 

A high-throughput method to globally study the organelle morphology in S. cerevisiae

Article DOI: 10.3791/1224
March 2nd, 2009

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Summary March 2nd, 2009

GFP-fusion proteins are widely used to visualize organelles by confocal microscopy. However, screening for mutations that affect the morphology of organelles generally requires individual mutagenesis and is time consuming. Here, we demonstrate a method to simultaneously incorporate organelle-GFP markers in almost 5,000 non-essential genes in yeast.

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