9.16

Ribosomal RNA Synthesis

3.9K views02:53 min

Non-coding RNAs, such as ribosomal RNAs, transfer RNAs, snoRNAs, and micro RNAs are the RNAs that do not code for proteins but are, themselves, the final product.

Ribosomal RNAs, the most abundant of the non-coding RNAs, are major structural components of the ribosomes. Eukaryotic ribosomes contain rRNAs of four types: 5S, 5.8S, 18S, and 28S.

Three of the four rRNA genes are encoded in a single DNA element, interspaced with transcribed spacer DNAs. The fourth rRNA, 5S rRNA, is encoded separately.

Within the nucleolus, the three rRNAs are transcribed together by RNA Polymerase I into a single large precursor rRNA called 45S precursor rRNA.

After transcription, the precursor rRNA is modified extensively. Two common modifications include nucleoside methylations and pseudouridylation.

In nucleoside methylation, the 2 prime hydroxyl position on the nucleotide sugar is methylated. In pseudouridylation, the base uridine isomerizes, generating a different form called ‘pseudouridine.’

These modifications are catalyzed by a category of RNA-protein complexes called ‘small nucleolar RNA-protein complexes’ or ‘snoRNPs’. Each snoRNP complex consists of a snoRNA and four or five proteins, including the enzyme that catalyzes the modification reaction.

The snoRNAs determine the sites of modification by base-pairing to the complementary sequences on the precursor rRNA. Then, they bring the associated RNA-modifying enzyme to the base to be modified.

Once chemically modified, the precursor rRNAs are cleaved into mature, individual 5.8S, 18S, and 28S rRNAs, which are then incorporated into the ribosomal subunits.

Outside the nucleolus, the 5S rDNA is transcribed by RNA Polymerase III as a 120-nucleotide long transcript. Unlike other ribosomal RNAs, the 5S rRNA remains unmodified. The unmodified 5S rRNA is then imported to the nucleolus to be assembled with the other ribosomal components.

Overview

Loading...

$$\rightleftharpoonup{xx}$$ $$\longleftharp{xx}$$, $$\longrightharp{xx}$$,

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.

Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors, which are imported from the cytoplasm. The extensive modification of ribosomal RNAs by snoRNPs is another distinct feature of eukaryotic ribosomes. Individually the modified bases may not seem to have a specific function collectively, all the modifications stabilize particular conformations of the ribosomal RNAs. Additionally, these modified bases are more concentrated in functional regions of the rRNA and regulate ribosomal activity in translation.

Both the rRNA modifications and processing of pre-rRNAs occur in the nucleolus because both these steps require components that are only found in the nucleolus. While the snoRNPs carry out chemical modification of the rRNA, other “nucleolar proteins” hydrolyze the transcribed “spacer RNA” in the precursor RNA to form the cleaved 18S, 5.8S and 28S rRNA. With the generation of the matured rRNAs, the free nucleolar proteins return to a nucleolar pool and are reutilized.

Cations such as Magnesium ions (Mg²⁺) play an important role in maintaining the structure of the ribosome. During experiments, the ribosome dissociates into subunits when Mg²⁺ is removed. Although the precise role of Mg²⁺ remains uncertain, it is plausible that the cations interact with ionized phosphates of the RNA to bridge the two ribosomal subunits.

Once ribosomal assembly is complete, some of the ribosomes associate with intracellular membranes, primarily the endoplasmic reticulum, whereas free ribosomes are distributed through the cytoplasm.

Transcript

Loading...

$$\rightleftharpoonup{xx}$$ $$\longleftharp{xx}$$, $$\longrightharp{xx}$$,

Non-coding RNAs, such as ribosomal RNAs, transfer RNAs, snoRNAs, and micro RNAs are the RNAs that do not code for proteins but are, themselves, the final product.

Ribosomal RNAs, the most abundant of the non-coding RNAs, are major structural components of the ribosomes. Eukaryotic ribosomes contain rRNAs of four types: 5S, 5.8S, 18S, and 28S.

Three of the four rRNA genes are encoded in a single DNA element, interspaced with transcribed spacer DNAs. The fourth rRNA, 5S rRNA, is encoded separately.

Within the nucleolus, the three rRNAs are transcribed together by RNA Polymerase I into a single large precursor rRNA called 45S precursor rRNA.

After transcription, the precursor rRNA is modified extensively. Two common modifications include nucleoside methylations and pseudouridylation.

In nucleoside methylation, the 2 prime hydroxyl position on the nucleotide sugar is methylated. In pseudouridylation, the base uridine isomerizes, generating a different form called ‘pseudouridine.’

These modifications are catalyzed by a category of RNA-protein complexes called ‘small nucleolar RNA-protein complexes’ or ‘snoRNPs’. Each snoRNP complex consists of a snoRNA and four or five proteins, including the enzyme that catalyzes the modification reaction.

The snoRNAs determine the sites of modification by base-pairing to the complementary sequences on the precursor rRNA. Then, they bring the associated RNA-modifying enzyme to the base to be modified.

Once chemically modified, the precursor rRNAs are cleaved into mature, individual 5.8S, 18S, and 28S rRNAs, which are then incorporated into the ribosomal subunits.

Outside the nucleolus, the 5S rDNA is transcribed by RNA Polymerase III as a 120-nucleotide long transcript. Unlike other ribosomal RNAs, the 5S rRNA remains unmodified. The unmodified 5S rRNA is then imported to the nucleolus to be assembled with the other ribosomal components.

Explore More Videos

Here Are The Most Relevant Keywords From The Text Ribosomal RNA Synthesis Ribosomal RNA