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09:51 min
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March 11, 2009
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We demonstrate the use of a constant-force extensometer to measure long-term extension (creep) of plant cell wall specimens induced by acidic buffers and expansin protein.
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MeasuringPlant Cell Wall ExtensionCreepAcidic PHAlpha-expansinAcid GrowthExtensibleLow PHPlant Hormone AuxinCell ElongationAcid-growth MechanismH+ PumpPlasma MembraneWall AcidificationExpansinsCell Wall-loosening ProteinsCell Turgor PressureEnlargementAcid-induced ExtensionCell Wall PolysaccharidesPectinsExpansin Activity AssayWall Materials Preparation TechniquesCucumber Hypocotyls
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Durachko, D. M., Cosgrove, D. J. Measuring Plant Cell Wall Extension (Creep) Induced by Acidic pH and by Alpha-Expansin. J. Vis. Exp. (25), e1263, doi:10.3791/1263 (2009).
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