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JoVE Journal
Biology

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In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells
 

In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells

Article DOI: 10.3791/1333
September 24th, 2009

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Summary September 24th, 2009

Here we present two techniques for manipulating gene expression in murine retinal ganglion cells (RGCs) by in utero and ex vivo electroporation. These techniques enable one to examine how alterations in gene expression affect RGC development, axon guidance, and functional properties.

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