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In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells
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In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells

In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells

DOI:
10.3791/1333-v

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25:06 min

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September 24, 2009

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, ,
1Departments of Pathology and Cell Biology, and Neuroscience,Columbia University College of Physicians and Surgeons, 2Department of Ophthalmology,Columbia University College of Physicians and Surgeons

Chapters

  • 00:05Title
  • 01:07Introduction
  • 01:46In utero Retinal Electroporation – Injecting and Electroporating DNA into E14.5 Murine Retinae in vivo
  • 08:40In utero Retinal Electroporation – Retrieval of Electroporated Retina at E18.5
  • 12:34Ex vivo Retinal Electroporation – Injecting and Electroporating DNA into E14.5 Murine Retinae in vitro
  • 18:49Ex vivo Retinal Electroporation – Harvesting and Plating of GFP+ Retinal Explants (2 days post-electroporation)
  • 23:33Representative Results from in utero and ex vivo Retinal Electroporations
  • 25:23Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present two techniques for manipulating gene expression in murine retinal ganglion cells (RGCs) by in utero and ex vivo electroporation. These techniques enable one to examine how alterations in gene expression affect RGC development, axon guidance, and functional properties.

Tags

In Utero ElectroporationEx Vivo ElectroporationGene ExpressionMouse Retinal Ganglion CellsRetinal DevelopmentAxon GuidanceRetinotopic OrganizationSynapse FormationTransgenic MiceGene ManipulationChick In Ovo ElectroporationNeonatal Mouse Pups ElectroporationAdult Rats ElectroporationEmbryonic Murine Retinae ElectroporationRGC DevelopmentAxon ProjectionsIn Vivo Electroporation

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