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Growth Factor-Coated Bead Placement on Dorsal Forebrain Explants
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Growth Factor-Coated Bead Placement on Dorsal Forebrain Explants

Growth Factor-Coated Bead Placement on Dorsal Forebrain Explants

DOI:
10.3791/134-v

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15:07 min

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January 30, 2007

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, ,
1Department of Developmental and Cell Biology,University of California, Irvine (UCI), 2Department of Physiology and Biophysics,University of California, Irvine (UCI), 3Department of Pathology,University of California, Irvine (UCI)

Chapters

  • 00:45Types of beads: Affi-Gel and Heparin Acrylic
  • 01:13Affi-Gel bead placement
  • 01:18Preparation of glass capillaries
  • 03:47Preparation of Affi-Gel beads
  • 09:32Affi-Gel bead placement on explant
  • 11:27Preparation of acrylic beads
  • 13:31Acrylic bead placement on explant
  • 14:32Example of obtained results

Summary

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This video demonstrates two methods for preparing and placing beads, which have been coated with growth factor, on explants of the developing cerebral cortex. These beads can be used to induce spatially restricted gene expression on developing neural tissue such as forebrain explants. Methods are given for using both Affi-Gel beads and heparin acryllic beads.

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Growth Factor-coated Bead PlacementDorsal ForebrainExplants

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