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0.22μmのSterivexフィルタおよび塩化セシウム密度勾配遠心分離からのDNA抽出
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Biology
DNA Extraction from 0.22 μM Sterivex Filters and Cesium Chloride Density Gradient Centrifugation
Please note that all translations are automatically generated.
Click here for the English version.
0.22μmのSterivexフィルタおよび塩化セシウム密度勾配遠心分離からのDNA抽出
DOI:
10.3791/1352-v
•
15:14 min
•
September 18, 2009
•
Jody J. Wright
,
Sangwon Lee
,
Elena Zaikova
,
David A. Walsh
,
Steven J. Hallam
1
Department of Microbiology and Immunology
,
University of British Columbia – UBC
Chapters
00:05
Title
00:08
Introduction
00:47
Cell Lysis and Digestion with Proteinase K
02:38
DNA Extraction
03:56
DNA Concentration and Washing
06:22
Determination of DNA Quality and Concentration
07:50
Cesium Chloride Gradient Centrifugation
14:13
Representative Gel to Check DNA Quality and Concentration
14:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我々は、精製のための塩化セシウム密度勾配遠心分離に続いて0.22μmのSterivexフィルタ、集中プランクトンのバイオマスからの高分子量のゲノムDNAの抽出のための方法を説明します。
Tags
DNA Extraction
High Molecular Weight Genomic DNA
Planktonic Biomass
Sterivex Filters
Storage/lysis Buffer
-80°C Storage
Cesium Chloride Density Gradient Centrifugation
Incubation Steps
Liberate DNA
Remove RNA
Phenol:Chloroform Extraction
Chloroform Extraction
Centrifugation
Protein Removal
Cell Membrane Removal
Aqueous DNA Extract
Buffer Exchange
Purification
Cesium Chloride Density Gradient
Sample Limitation
Protocol Time
Centrifugation Equipment
Hybridization Ovens
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