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0.22μmのSterivexフィルタおよび塩化セシウム密度勾配遠心分離からのDNA抽出
JoVE Journal
Biology
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JoVE Journal Biology
DNA Extraction from 0.22 μM Sterivex Filters and Cesium Chloride Density Gradient Centrifugation
DOI:

15:14 min

September 18, 2009

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Chapters

  • 00:05Title
  • 00:08Introduction
  • 00:47Cell Lysis and Digestion with Proteinase K
  • 02:38DNA Extraction
  • 03:56DNA Concentration and Washing
  • 06:22Determination of DNA Quality and Concentration
  • 07:50Cesium Chloride Gradient Centrifugation
  • 14:13Representative Gel to Check DNA Quality and Concentration
  • 14:40Conclusion

Summary

Automatic Translation

我々は、精製のための塩化セシウム密度勾配遠心分離に続いて0.22μmのSterivexフィルタ、集中プランクトンのバイオマスからの高分子量のゲノムDNAの抽出のための方法を説明します。

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