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In vivo Ca2+– Imaging of Mushroom Body Neurons During Olfactory Learning in the Honey Bee
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In vivo Ca2+– Imaging of Mushroom Body Neurons During Olfactory Learning in the Honey Bee

In vivo Ca2+– Imaging of Mushroom Body Neurons During Olfactory Learning in the Honey Bee

English

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13,860 Views

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10:27 min

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August 18, 2009

DOI:

10.3791/1353-v

DOI:
10.3791/1353-v

•

10:27 min
August 18, 2009

•

4 Views
, ,
1Institut für Biologie – Neurobiologie,Freie Universität Berlin, 2Institut für Biologie – Neurobiologie,Free University Berlin – Freie Universitaet Berlin

Summary

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Bees can be conditioned in an appetitive olfactory learning paradigm (PER-conditioning). Using odors as stimuli, we established a method in which behavior is recorded while simultaneously Calcium Imaging is used to measure odor evoked activity in mushroom body neurons in vivo.

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Cite this Article

Haehnel, M., Froese, A., Menzel, R. In vivo Ca2+- Imaging of Mushroom Body Neurons During Olfactory Learning in the Honey Bee. J. Vis. Exp. (30), e1353, doi:10.3791/1353 (2009).

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