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移植嵌合斑马鱼胚胎生成
JoVE Journal
Biology
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JoVE Journal
Biology
Generating Chimeric Zebrafish Embryos by Transplantation
Please note that all translations are automatically generated.
Click here for the English version.
移植嵌合斑马鱼胚胎生成
DOI:
10.3791/1394-v
•
21:01 min
•
July 17, 2009
•
Hilary A. Kemp
,
Amanda Carmany-Rampey
,
Cecilia Moens
1
HHMI and Division of Basic Sciences
,
Fred Hutchinson Cancer Research Center – FHCRC
Chapters
00:05
Title
00:13
Introduction
01:12
Injecting Zebrafish Embryos at the 1-cell Stage
06:15
Preparing the Transplantation Rig and Needle
08:31
Transplanting Cells between Bastula Stage Embryos
13:00
Transplanting Primordial Germ Cells at the Blastula Stage
15:02
Transplating Cells Between Gastrula Stage Embryos
18:46
Representative Results/ Outcome
20:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在囊胚或原肠胚阶段有针对性的嵌合体斑马鱼的胚胎移植产生由的一步一步的指导。
Tags
Chimeric Embryos
Zebrafish
Transplantation
Chimera Analysis
Mosaic Chimeras
Donor Embryo
Host Embryo
Lineage Marker
Fluorescent Dye
Transplantation Site
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