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Chapters
- 00:32Materials
- 01:10Picking larvae
- 01:30Dissecting larvae
- 02:53Tissue fixation
- 04:11Removal of discs on microscopic slide
- 07:07Examples of applications
This protocol demonstrates the dissection technique used for isolating imaginal discs from drosophila larvae at the 3rd instar stage. Methods for fixing the tissue after saying and removing the wing, leg, and eye discs are demonstrated directly on a microscope slide for subsequent visualization.
