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Biology

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Fast and Sensitive Colloidal Coomassie G-250 Staining for Proteins in Polyacrylamide Gels
 

Fast and Sensitive Colloidal Coomassie G-250 Staining for Proteins in Polyacrylamide Gels

Article doi: 10.3791/1431
August 3rd, 2009

Summary August 3rd, 2009

This video shall popularize a colloidal Coomassie G-250 staining protocol according to Kang et al. for the detection of average 4 ng protein in gels. The staining is completed within 2 hours and without any effort. We routinely use Kang's protocol for analytical purposes in gel-based proteomics.

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