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Chapters
- 00:05Title
- 00:35Introduction
- 01:09Fluorescent Labeling of cDNA
- 02:55Cleanup and Precipitation of the Labeled cDNA
- 05:49Hybridization
- 08:47Post-hybridization Washes and Scanning of Microarray
- 11:32Representative Results
- 12:40Conclusion
Gene microarrays are powerful tools in gene expression profiling at a genome-wide level. This technology has application in a variety of biological disciplines including developmental biology and toxicology. In this video, we detail a protocol for global gene expression analysis using a comprehensive oligonucleotide microarray platform for the zebrafish.
Tags
Gene Expression AnalysisZebrafish Oligonucleotide Microarray PlatformGene Microarray TechnologyQuantitative MeasurementGene Expression ProfilingTranscript LevelsGenome-wide BasisBiological DisciplinesDevelopmental StageDisease StateToxic ResponsesZebrafish Expression Microarray PlatformProbesTargetsCDNAGenomic InformationGenomic DatabasesEnsemblVEGAUCSCZFINTranscriptome CoverageRoche NimbleGenFluorescent LabelingHybridizationArray ScanningSignal Acquisition
