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Journal
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Biology
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脂肪酸定量磷酸化刺激
酿酒酵母</em
JoVE Journal
Biology
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JoVE Journal
Biology
Quantitative Phosphoproteomics in Fatty Acid Stimulated
Saccharomyces cerevisiae
Please note that all translations are automatically generated.
Click here for the English version.
脂肪酸定量磷酸化刺激
酿酒酵母</em
DOI:
10.3791/1474-v
•
15:41 min
•
October 12, 2009
•
Ramsey A. Saleem
,
John D. Aitchison
1
Institute for Systems Biology
Chapters
00:00
Title
00:44
Introduction
01:02
Growing Isotopically Light and Isotopically Heavy Yeast Cells
03:06
Lysing Cells
06:35
Isolation and Fractionation of Peptides
06:38
Alkylation and Reduction
10:32
Desalting
11:54
Peptide Fractionation
13:11
Enrichment of Phosphopeptides
14:51
Representative Results
15:14
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
描述了一个定量的磷酸化过程,使用cryolysis,尿素solubilziation,HILIC分馏和富集磷酸化肽的iMac。
Tags
Quantitative Phosphoproteomics
Fatty Acid Stimulation
Saccharomyces Cerevisiae
Growth And Stimulation
Oleate
Isotopically Heavy And Light Cells
Cryolysis Procedure
Ball Mill Grinder
Urea Solubilization
Metabolically Inactive State
Phosphorylation Preservation
Preventing Reorientation
Cell Lysis
Reduction
Alkylation
Trypsin Digestion
Desalting On C18 Columns
Sample Complexity Reduction
Fractionation Using Hydrophilic Interaction Chromatography (HILIC)
Phosphopeptides Elution Profile
Immobilized Metal Chromatography
Charge-based Affinities
Mass Spectrometry Analysis
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