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September 07, 2009
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Summary
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We present an in vitro, two-color fluorescence assay to visualize the fusion of single virus particles with a fluid target bilayer. By labeling viral particles with fluorophores that differentially stain the viral membrane and its interior, we are able to monitor the kinetics of hemifusion and pore formation.
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Cite this Article
Floyd, D. L., Harrison, S. C., van Oijen, A. M. Method for Measurement of Viral Fusion Kinetics at the Single Particle Level. J. Vis. Exp. (31), e1484, doi:10.3791/1484 (2009).
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