Culture of Mouse Neural Stem Cell Precursors

Journal

Biology

JoVE Journal
Biology

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JoVE Journal Biology

Culture of Mouse Neural Stem Cell Precursors

DOI:

10.3791/152-v

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27:44 min

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February 25, 2007

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D. Spencer Currle, Jia Sheng Hu, Aaron Kolski-Andreaco, Edwin S. Monuki

¹Department of Developmental and Cell Biology,University of California, Irvine (UCI), ²Department of Pathology,University of California, Irvine (UCI), ³Department of Physiology and Biophysics,University of California, Irvine (UCI)

Chapters

00:36Preparing plate
05:10Instruments
05:39Dissection of E12.5 mouse
07:55Removal of embryos
13:20Isolation of cortex
18:56Obtaining cell suspension
23:21Plating neural precursors

Summary

Automatic Translation

This video describes the method used for isolation of neuroprecursors from the developing cortex of embryonic mice. The procedure for removing embryos from the uterus, dissecting the cortical tissue, and digesting the isolated cerebral cortex is shown.

Culture of Mouse Neural Stem Cell Precursors

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