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Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells
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Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

DOI:
10.3791/1524-v

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12:17 min

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October 28, 2009

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, ,
1Stem Cell Research Unit,Medical University of Graz, Austria

Chapters

  • 00:00Title
  • 00:41Introduction
  • 01:18Isolation of Endothelial Colony-Forming Progenitor Cells (ECFCs)
  • 05:46Harvesting the ECFCs
  • 08:19ECFC Expansion
  • 10:57Representative ECFC Preparations
  • 11:45Conclusion

Summary

Automatic Translation

Automatic Translation

Endothelial colony forming progenitor cells (ECFCs) are a promising tool to study vascular homeostasis and repair.1,2 This paper introduces a novel animal-serum free method for isolation and expansion of ECFC from heparinised adult human peripheral blood with pooled human platelet lysate (pHPL) diminishing the risk of anti-bovine immunisation.

Tags

IsolationLarge Scale ExpansionAdult HumanEndothelial Colony Forming Progenitor CellsRecovery StrategyHeparinized BloodPeripheral BloodPHPLBovine Serum AntigensFlow CytometryImmunohistochemistryIn Vitro FunctionalityVascular StructuresMatrigel AssaySubcutaneous InjectionMouse ModelPerfused VesselsCryopreservationProliferationGenomic StabilityAnimal-protein Free Isolation

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