Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

Nicole A. Hofmann; Andreas Reinisch; Dirk Strunk

doi:10.3791/1524

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

Full Text

11,298 Views

12:17 min

October 28, 2009

DOI: 10.3791/1524-v

Nicole A. Hofmann¹, Andreas Reinisch¹, Dirk Strunk¹

¹Stem Cell Research Unit,Medical University of Graz, Austria

Endothelial colony forming progenitor cells (ECFCs) are a promising tool to study vascular homeostasis and repair.^1,2 This paper introduces a novel animal-serum free method for isolation and expansion of ECFC from heparinised adult human peripheral blood with pooled human platelet lysate (pHPL) diminishing the risk of anti-bovine immunisation.

By simply culturing the total blood with animal serum free optimized medium. In a 75 square centimeter flask, E CFCs adhere to the flask surface to remove most of the red and white blood cells. Wash the flask on day one and eliminate the remaining cells by changing the total medium on day five.

Keep screening for colonies every other day, or if convenient three times per week. And you should observe the first colonies around day 12. Hi, my name is Nicole Hoffman from the lab of at the stem Cell Research Unit of the Medical University grots.

View the full transcript and gain access to thousands of scientific videos