Mouse Model Injection
Mouse Model Injection - Related Videos
03:05
Establishing a Focal Demyelination Mouse Model via a Two-Point Injection of Lysophosphatidylcholine
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2025
This video demonstrates the generation of a focal demyelination mouse model using a two-point Lysophosphatidylcholine (LPC) injection. The skull of an anesthetized mouse is exposed. The injection coordinates are determined, and a hole is drilled to enable LPC injections at targeted sites. LPC induces focal demyelination of neurons via inflammation, while a two-point injection ensures its sustainability.
07:43
Intracarotid Cancer Cell Injection to Produce Mouse Models of Brain Metastasis
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Cited by 32 •
2017
Brain metastasis has become an urgent unmet medical need as its incidence has increased while therapeutic options have remained palliative. Creating experimental animal models of brain metastasis via intracarotid arterial injection of cancer cells facilitates mechanistic studies of the disease biology and evaluation of novel intervention regimens.
02:59
Stereotactic Injection of Hydrogel into a Post-Stroke Mouse Model
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2025
Source: Wilson, K.L., et al. Injection of Hydrogel Biomaterial Scaffolds to The Brain After Stroke. J. Vis. Exp. (2020)This video demonstrates the stereotaxic injection of a hydrogel into the stroke core of a post-stroke mouse, highlighting its role in scaffold formation that mimics the extracellular matrix, promotes cellular infiltration, and supports angiogenesis, underscoring its neurotherapeutic potential in stroke recovery.
04:58
Brain Metastasis Mouse Model: A Method to Generate Brain Metastasis Mouse Model by Intracarotid Cancer Cell Injection
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2025
This video demonstrates the establishment of a brain metastasis mouse model by intracarotid injection of fluorescently labeled cancer cells. The mouse model can be used to study the effects of novel therapeutics and the underlying physiological mechanisms.
02:43
Tumor Implantation and Injection of a Tumor Vaccine in a Mouse Model
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2025
This video demonstrates a cell-based tumor vaccination assay. A mouse, intradermally injected with B16-F10 melanoma cells for tumor formation, is vaccinated with B16-F10 cells secreting Flt3L cytokine near the developed tumor. The secreted Flt3L from the vaccine promotes the maturation and proliferation of dendritic cells that then present tumor antigens to T cells in lymph nodes, triggering a targeted anti-tumor immune response.
FAQs
The selected route determines how a substance enters tissues, reaches the circulation, and produces its biological effect. Intravenous, intraperitoneal, subcutaneous, and intramuscular administration therefore may not produce equivalent experimental outcomes, even when the same compound is delivered. Matching the route to the study objective helps researchers evaluate biological responses under controlled and interpretable conditions.
Accurate dosing helps ensure that differences between experimental groups reflect the substance being studied rather than inconsistent administration. Sterile technique supports reliable results and contributes to animal welfare by reducing avoidable complications associated with contamination. Together, these practices improve reproducibility, making biological effects, treatment responses, and safety findings easier to interpret.
Each route provides a different pattern of tissue entry, access to the circulation, and resulting effect. Researchers may therefore select intravenous, intraperitoneal, subcutaneous, or intramuscular administration according to the biological question and the response they need to study. Route selection is especially important when comparing treatment efficacy, disease mechanisms, or safety outcomes.
Researchers should establish the administration route, the intended dose, and the sterile conditions before beginning injections. Applying the same approach across relevant experimental groups helps reduce avoidable variation and strengthens comparisons. Standardization is valuable when assessing whether observed differences arise from treatment effects, biological mechanisms, or inconsistent delivery.
This technique supports studies of disease modeling, pharmacology, toxicology, immunology, and gene or cell delivery. In these settings, researchers can introduce a substance and then examine biological processes, disease mechanisms, therapeutic responses, or safety. Its broad use makes it relevant to investigations ranging from understanding pathology to evaluating potential treatments before further research.
Injection studies can help researchers evaluate treatment efficacy, biological mechanisms, and safety in living mice. Results may show how a delivered substance affects a disease model or produces a therapeutic response under a defined administration route and dose. These findings provide experimental evidence that can inform decisions about advancing a treatment or biological approach to further research.