Name: Dissection of Hippocampal Dentate Gyrus from Adult Mouse (Video) | JoVE Journal
Uploaded: 2009-11-17T20:30:00.000+00:00
Duration: 7 min 42 s
Description: Learn a precise dissection technique for isolating the dentate gyrus from adult mouse hippocampus using a stereomicroscope. This method enables collection of intact samples for transcriptomic and proteomic analyses.

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5 minutes

We thank Dr. Yoko Nabeshima at Kyoto University for her instruction on the dissection technique and Ms. Aki Miyakawa at Fujita Health University for her support to film. This work was supported by the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation, a Grant-in-Aid for Scientific Research on Priority Areas -Integrative Brain Research (Shien)- from MEXT in Japan, and by a Grant-in-Aid from CREST of the Japan Science and Technology Agency.

Dissection of hippocampal dentate gyrus
<ol>
 <li>In a deeply anesthetized mouse, carefully dissect the brain out from the skull and place it into ice-cold phosphate-buffered saline (PBS).</li>
 <li>In a Petri dish containing ice-cold PBS, cut the brain along the longitudinal fissure of the cerebrum using a surgical knife, and cut off the regions posterior to lambda (midbrain, hindbrain, and cerebellum).</li>
 <li>Place the cerebral hemisphere medial side up and, using forceps, carefully remove the diencephalon (thalamus and hypothalamus) under a dissection microscope. This will expose the medial side of the hippocampus, allowing for visualization of the dentate gyrus. The dentate gyrus is distinguishable from Ammon's horn by the gaps between them. Injury to the hippocampus or surrounding area will make it more difficult to isolate the dentate gyrus.</li>
 <li>Insert a sharp needle-tip (e.g., 27-gauge needle) into each side of the dentate gyrus (boundaries of the dentate gyrus and Ammon's horn; Figure 1), and slide the needles superficially along the septo-temporal axis of hippocampus to isolate the dentate gyrus.</li>
 <li>Pick up the isolated dentate gyrus using a needle or forceps and place it in a sample tube. The thus-obtained dentate gyrus tissue sample can be used immediately for any assay or stored in a deep-freezer for later use.</li>
 <li>Isolate the dentate gyrus from the other cerebral hemisphere using the same method.</li>
</ol>
Quantitative real-time PCR
The dentate gyrus was isolated using the above-mentioned method and the remaining hippocampus was dissected out as the Ammon's horn sample from wild-type mice. Real-time PCR of beta-actin, TDO2, Dsp, Mrg1b and Tyro3 were performed with the dentate gyrus and the Ammon's horn samples as described previously1. Primers 5'-CTGGCGAGATCACGATGACG and 5'-AAGCTACGCTGTTGTCTAACC were used for Mrg1b, and GCCTCCAAATTGCCCGTCA and 5'-CCAGCACTGGTACATGAGATCA for Tyro3. 
Microarray analysis
Microarray experiments were performed with male wild-type mice and mice heterozygous for the alpha-isoform of calcium/calmodulin-dependent protein kinase II (alpha-CaMKII+/- mice) as described previously1. Briefly, RNA isolated from the whole hippocampus or dentate gyrus of wild-type and mutant mice was hybridized with a Mouse Genome 430 2.0 Array (Affymetrix, Santa Clara, CA), and each GeneChip was scanned by an Affymetrix GeneChip Scanner 3000 (GCS3000). GeneChip analysis was performed with Microarray Analysis Suite version 5.0.

<ol><li>Yamasaki, N., Maekawa, M., Kobayashi, K., Kajii, Y., Maeda, J., Soma, M., Takao, K., Tanda, K., Ohira, K., Toyama, K., Kanzaki, K., Fukunaga, K., Sudo, Y., Ichinose, H., Ikeda, M., Iwata, N., Ozaki, N., Suzuki, H., Higuchi, M., Suhara, T., Yuasa, S., Miyakawa, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Alpha-CaMKII+deficiency+causes+immature+dentate+gyrus%2C+a+novel+candidate+endophenotype+of+psychiatric+disorders%5BTitle%5D)+AND+%22Mol.+Brain%22%5BJournal%5D)">Alpha-CaMKII deficiency causes immature dentate gyrus, a novel candidate endophenotype of psychiatric disorders.</a> Mol. Brain. 1, 6-6 (2008).</li>
<li>Insausti, A. M., Megias, M., Crespo, D., Cruz-Orive, L. M., Dierssen, M., Vallina, I. F., Insausti, R., Florez, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Hippocampal+volume+and+neuronal+number+in+Ts65Dn+mice%3A+a+murine+model+of+Down+syndrome%5BTitle%5D)+AND+%22Neurosci.+Lett%22%5BJournal%5D)">Hippocampal volume and neuronal number in Ts65Dn mice: a murine model of Down syndrome.</a> Neurosci. Lett. 253, 175-175 (1998).</li>
<li>Redwine, J. M., Kosofsky, B., Jacobs, R. E., Games, D., Reilly, J. F., Morrison, J. H., Young, W. G., Bloom, F. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Dentate+gyrus+volume+is+reduced+before+onset+of+plaque+formation+in+PDAPP+mice%3A+a+magnetic+resonance+microscopy+and+stereologic+analysis%5BTitle%5D)+AND+%22Proc.+Natl.+Acad.+Sci.+U.S.A%22%5BJournal%5D)">Dentate gyrus volume is reduced before onset of plaque formation in PDAPP mice: a magnetic resonance microscopy and stereologic analysis.</a> Proc. Natl. Acad. Sci. U.S.A. 100, 1381-1381 (2003).</li>
<li>Lein, E. S., Zhao, X., Gage, F. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Defining+a+molecular+atlas+of+the+hippocampus+using+DNA+microarrays+and+high-throughput+in+situ+hybridization%5BTitle%5D)+AND+%22J.+Neurosci%22%5BJournal%5D)">Defining a molecular atlas of the hippocampus using DNA microarrays and high-throughput in situ hybridization.</a> J. Neurosci. 24, 3879-3879 (2004).</li>
<li>Matsuo, N., Yamasaki, N., Ohira, K., Takao, K., Toyama, K., Eguchi, M., Yamaguchi, S., Miyakawa, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Neural+activity+changes+underlying+the+working+memory+deficit+in+alpha-CaMKII+heterozygous+knockout+mice%5BTitle%5D)+AND+%22Front.+Behav.+Neurosci%22%5BJournal%5D)">Neural activity changes underlying the working memory deficit in alpha-CaMKII heterozygous knockout mice.</a> Front. Behav. Neurosci. 3, 20-20 (2009).</li>
<li>Franklin, K. B. J., Paxinos, G. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aFranklin%2C+KBJ+%22The+Mouse+Brain+in+Stereotaxic+Coordinates%22">The Mouse Brain in Stereotaxic Coordinates</a>. , Academic Press Inc.. San Diego. (1997).</li>
</ol>

Animal experiments were performed in accordance with the National Institute of Health Guide for Care and Use of Laboratory Animals, and approved by the animal care and use committee at Fujita Health University.

The dentate gyrus occupies approximately 25% to 30% of the volume of the hippocampal formation2,3. It has a unique cell composition and plays crucial roles in various brain functions. Therefore, techniques to isolate the dentate gyrus are useful for analyzing the events that occur specifically in this region.
Here, we demonstrated a procedure to efficiently dissect the dentate gyrus from adult mouse hippocampus and confirmed the precision of the technique. First, histologic study revealed that the dentate gyrus was separated without contamination by other regions (Figure 1), indicating that a pure dentate gyrus sample can be prepared.
Second, we confirmed that the dissected tissue is dentate gyrus by conducting real-time PCR of dentate gyrus-specific genes, TDO2 and Dsp, and Ammon's horn enriched genes, Mrg1b and Tyro34 (Figure 2). The mRNA expressions of TDO2 (p=0.000023; n's=4 and 4, respectively) and Dsp (p=0.0000030; n's=4 and 4, respectively) in the dentate gyrus samples were detected at obviously higher levels, whereas Mrg1b (p=0.000080; n's=4 and 4, respectively) and Tyro3 (p=0.00017; n's=4 and 4, respectively) were lower levels, than those in the Ammon's horn samples. Beta-actin expression levels did not differ in these samples (p=0.11; n's=4 and 4, respectively). Thus, we could check whether or not the dentate gyrus was accurately dissected out by conducting such simple real-time PCR experiments.
Third, to assess the usefulness of this dissection method, we compared the mRNA expression level of whole hippocampus with that of dentate gyrus. Whole hippocampus and dentate gyrus obtained from wild-type (n's=9 and 4, respectively) and alpha-CaMKII+/- mice (n's=18 and 4, respectively) were processed for microarray analysis, and for all genes scored, the fold-change was calculated by dividing the mutant value by the wild-type value. The results indicated that the changes in mRNA expression, especially of dentate gyrus-specific molecules such as Dsp and TDO2, were detected with up to a 5-fold increase in sensitivity in dentate gyrus samples compared to whole hippocampal samples (Table 1). We previously demonstrated that alpha-CaMKII+/- mice exhibit behaviors related to human psychiatric disorders such as working memory deficits and an exaggerated infradian rhythm1,5. Furthermore, morphologic and electrophysiologic features of the dentate gyrus neurons in mutant mice are strikingly similar to those of immature dentate gyrus neurons in normal rodents, indicating that the neurons in these mutant mice fail to develop to maturity1. The immature dentate gyrus and down-regulated expression of Dsp and TDO2 mRNA in alpha-CaMKII+/- mice are consistent with the finding that Dsp and TDO2 can be used as markers of mature granule cells in the dentate gyrus (Ohira et al., unpublished data).
Taken together, this convenient and accurate dissection technique can be reliably used for studies focused on the dentate gyrus. Dentate gyrus tissue obtained using this method is applicable to other types of analyses as well, including proteomic and cell biology analyses.
<img src="/files/ftp_upload/1543/1543fig1.jpg" alt="Histology brain section with diagram; microscopy; structure analysis; marked regions; scientific study." data-altupdatedat="1787630408000" data-alt="figure 1"> Figure 1. Verification of the isolated dentate gyrus by histologic study. A coronal section of the brain after isolating dentate gyrus was processed for Nissl staining (left panel), and a schematic diagram adapted from the mouse brain atlas6 represents the approximately the same level of the section shown in the left panel (right panel). Arrows indicate the directions of the needle-tip insertion. Scale bar, 1 mm.
<img src="/files/ftp_upload/1543/1543fig2.jpg" alt="Gene expression comparison, bar chart shows expression levels in Dentate gyrus vs Ammon's horn." data-altupdatedat="1787630408000" data-alt="figure 2"> Figure 2. Verification of the isolated dentate gyrus by real-time PCR. The dentate gyrus and the Ammon s horn obtained from four wild-type mice were processed for real-time PCR of beta-actin, TDO2, Dsp, Mrg1b and Tyro3. Results are presented as means ± SEM. For statistical analysis, Student s t test was employed, and p values are followed: beta-actin, p=0.11; TDO2, p=0.000023 (**1); Dsp, p=0.0000030 (**2); Mrg1b, p=0.000080 (**3); and Tyro3, p=0.00017 (**4).
Table 1. Microarray analysis of whole hippocampus and dentate gyrus. Genes differentially expressed in dentate gyrus and whole hippocampus of alpha-CaMKII+/- mice were determined by calculating the fold-change from that detected in wild-type mice. Data were analyzed for statistical significance using the Student s t test between wild-type and alpha-CaMKII+/- mice. Among the genes whose expression exhibited p&lt;0.05 in the dentate gyrus of alpha-CaMKII+/- mice compared to that of wild-type mice, the top 50 genes are listed. Note that the numbers of samples for dentate gyrus are much less than those for whole hippocampus. AffyID, Affymetrix probe identifier; CKII, alpha-CaMKII+/- mice; WT, wild-type mice. 
<table border="1">
 <tbody>
 <tr>
 <td> </td>
 <td> </td>
 <td> </td>
 <td> </td>
 <td colspan="2"> Dentate gyrus (p &lt;0.05) WT: n=4, CKII+/-: n=4</td>
 <td> </td>
 <td colspan="2"> Whole hippocampus WT: n=9, CKII+/-: n=18</td>
 </tr>
 <tr>
 <td>Gene Title</td>
 <td>Genebank</td>
 <td>AffyID</td>
 <td> </td>
 <td>Fold change</td>
 <td>p value</td>
 <td> </td>
 <td>Fold change</td>
 <td>p value</td>
 </tr>
 <tr>
 <td>desmoplakin</td>
 <td>AV297961</td>
 <td>1435494_s_at</td>
 <td> </td>
 <td>0.011018913</td>
 <td>7.02694E-06</td>
 <td> </td>
 <td>0.037021003</td>
 <td>1.86126E-13</td>
 </tr>
 <tr>
 <td>desmoplakin</td>
 <td>AV297961</td>
 <td>1435493_at</td>
 <td> </td>
 <td>0.014369734</td>
 <td>7.86747E-06</td>
 <td> </td>
 <td>0.04232106</td>
 <td>1.00579E-12</td>
 </tr>
 <tr>
 <td>tryptophan 2,3-dioxygenase</td>
 <td>AI098840</td>
 <td>1419093_at</td>
 <td> </td>
 <td>0.020986484</td>
 <td>5.23546E-09</td>
 <td> </td>
 <td>0.101037776</td>
 <td>4.14823E-13</td>
 </tr>
 <tr>
 <td>nephronectin</td>
 <td>AA223007</td>
 <td>1452106_at</td>
 <td> </td>
 <td>0.075479901</td>
 <td>1.05191E-08</td>
 <td> </td>
 <td>0.234001154</td>
 <td>1.66301E-15</td>
 </tr>
 <tr>
 <td>nephronectin</td>
 <td>AA223007</td>
 <td>1452107_s_at</td>
 <td> </td>
 <td>0.079457767</td>
 <td>1.40433E-07</td>
 <td> </td>
 <td>0.177974715</td>
 <td>3.9758E-12</td>
 </tr>
 <tr>
 <td>thyrotropin releasing hormone receptor</td>
 <td>M59811</td>
 <td>1449571_at</td>
 <td> </td>
 <td>0.103105815</td>
 <td>0.003093796</td>
 <td> </td>
 <td>0.801412732</td>
 <td>0.283994361</td>
 </tr>
 <tr>
 <td>ryanodine receptor 1, skeletal muscle</td>
 <td>X83932</td>
 <td>1427306_at</td>
 <td> </td>
 <td>0.104825517</td>
 <td>3.38513E-07</td>
 <td> </td>
 <td>0.650685017</td>
 <td>0.000308462</td>
 </tr>
 <tr>
 <td>nescient helix loop helix 1</td>
 <td>NM_010916</td>
 <td>1419533_at</td>
 <td> </td>
 <td>9.431896</td>
 <td>6.7979E-06</td>
 <td> </td>
 <td>4.078815314</td>
 <td>5.27E-11</td>
 </tr>
 <tr>
 <td>copine family member IX</td>
 <td>BB274531</td>
 <td>1454653_at</td>
 <td> </td>
 <td>9.159157</td>
 <td>7.99492E-06</td>
 <td> </td>
 <td>1.797304153</td>
 <td>0.000296375</td>
 </tr>
 <tr>
 <td>doublecortin-like kinase 3</td>
 <td>BB326709</td>
 <td>1436532_at</td>
 <td> </td>
 <td>0.109336662</td>
 <td>1.95278E-07</td>
 <td> </td>
 <td>0.56697229</td>
 <td>2.62633E-08</td>
 </tr>
 <tr>
 <td>calpain 3</td>
 <td>AF127766</td>
 <td>1426043_a_at</td>
 <td> </td>
 <td>0.111269769</td>
 <td>8.07053E-06</td>
 <td> </td>
 <td>0.370956608</td>
 <td>2.04421E-14</td>
 </tr>
 <tr>
 <td>Adult male corpus striatum cDNA, RIKEN full-length enriched library, clone:C030023B07 product:unclassifiable, full insert sequence</td>
 <td>BB357628</td>
 <td>1460043_at</td>
 <td> </td>
 <td>0.118712341</td>
 <td>6.16926E-07</td>
 <td> </td>
 <td>0.682339204</td>
 <td>2.33001E-06</td>
 </tr>
 <tr>
 <td>collagen and calcium binding EGF domains 1</td>
 <td>AV264768</td>
 <td>1437385_at</td>
 <td> </td>
 <td>0.124043978</td>
 <td>3.65669E-05</td>
 <td> </td>
 <td>0.488394112</td>
 <td>4.05538E-06</td>
 </tr>
 <tr>
 <td>amyloid beta (A4) precursor protein-binding, family A, member 2 binding protein</td>
 <td>AK013520</td>
 <td>1431946_a_at</td>
 <td> </td>
 <td>7.7986307</td>
 <td>1.2098E-06</td>
 <td> </td>
 <td>2.099164713</td>
 <td>1.67047E-06</td>
 </tr>
 <tr>
 <td>calbindin-28K</td>
 <td>BB177770</td>
 <td>1456934_at</td>
 <td> </td>
 <td>0.130255444</td>
 <td>3.32186E-06</td>
 <td> </td>
 <td>0.572605751</td>
 <td>1.99157E-10</td>
 </tr>
 <tr>
 <td>Transcribed locus</td>
 <td>AV328597</td>
 <td>1443322_at</td>
 <td> </td>
 <td>0.133290835</td>
 <td>5.43583E-06</td>
 <td> </td>
 <td>0.562767164</td>
 <td>7.56544E-06</td>
 </tr>
 <tr>
 <td>neuropeptide Y receptor Y2</td>
 <td>NM_008731</td>
 <td>1417489_at</td>
 <td> </td>
 <td>0.135319609</td>
 <td>0.000113407</td>
 <td> </td>
 <td>0.781498474</td>
 <td>0.00394504</td>
 </tr>
 <tr>
 <td>ras responsive element binding protein 1</td>
 <td>BE197381</td>
 <td>1428657_at</td>
 <td> </td>
 <td>0.138235114</td>
 <td>7.93691E-07</td>
 <td> </td>
 <td>0.651220705</td>
 <td>2.94209E-05</td>
 </tr>
 <tr>
 <td>glial cell line derived neurotrophic factor family receptor alpha 2</td>
 <td>BB284482</td>
 <td>1433716_x_at</td>
 <td> </td>
 <td>0.139062563</td>
 <td>2.35371E-06</td>
 <td> </td>
 <td>0.669544709</td>
 <td>0.000214146</td>
 </tr>
 <tr>
 <td>preproenkephalin 1</td>
 <td>M13227</td>
 <td>1427038_at</td>
 <td> </td>
 <td>6.9850435</td>
 <td>2.39074E-08</td>
 <td> </td>
 <td>1.766018828</td>
 <td>0.000250501</td>
 </tr>
 <tr>
 <td>RIKEN cDNA 1810010H24 gene</td>
 <td>BI729991</td>
 <td>1428809_at</td>
 <td> </td>
 <td>6.8658915</td>
 <td>1.88516E-05</td>
 <td> </td>
 <td>2.77573142</td>
 <td>6.81865E-09</td>
 </tr>
 <tr>
 <td>ryanodine receptor 1, skeletal muscle</td>
 <td>BG793713</td>
 <td>1457347_at</td>
 <td> </td>
 <td>0.151364292</td>
 <td>3.35612E-05</td>
 <td> </td>
 <td>0.503144617</td>
 <td>4.32907E-05</td>
 </tr>
 <tr>
 <td>protocadherin 21</td>
 <td>NM_130878</td>
 <td>1418304_at</td>
 <td> </td>
 <td>0.152671849</td>
 <td>8.57783E-06</td>
 <td> </td>
 <td>0.670714726</td>
 <td>1.56309E-05</td>
 </tr>
 <tr>
 <td>cornichon homolog 3 (Drosophila)</td>
 <td>NM_028408</td>
 <td>1419517_at</td>
 <td> </td>
 <td>0.153724144</td>
 <td>8.90755E-06</td>
 <td> </td>
 <td>0.95780695</td>
 <td>0.661055608</td>
 </tr>
 <tr>
 <td>harakiri, BCL2 interacting protein (contains only BH3 domain)</td>
 <td>BQ175572</td>
 <td>1439854_at</td>
 <td> </td>
 <td>0.154284407</td>
 <td>2.0118E-05</td>
 <td> </td>
 <td>0.56516812</td>
 <td>4.86925E-09</td>
 </tr>
 <tr>
 <td>carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 9</td>
 <td>AK017407</td>
 <td>1431897_at</td>
 <td> </td>
 <td>0.155238951</td>
 <td>5.37423E-06</td>
 <td> </td>
 <td>1.14910007</td>
 <td>0.215637733</td>
 </tr>
 <tr>
 <td>calpain 3</td>
 <td>AI323605</td>
 <td>1433681_x_at</td>
 <td> </td>
 <td>0.160871988</td>
 <td>1.07655E-05</td>
 <td> </td>
 <td>0.477164757</td>
 <td>1.33753E-11</td>
 </tr>
 <tr>
 <td>zinc finger, CCHC domain containing 5</td>
 <td>BQ126004</td>
 <td>1437355_at</td>
 <td> </td>
 <td>0.161812078</td>
 <td>3.08262E-06</td>
 <td> </td>
 <td>0.421252632</td>
 <td>0.01152969</td>
 </tr>
 <tr>
 <td>loricrin</td>
 <td>NM_008508</td>
 <td>1448745_s_at</td>
 <td> </td>
 <td>0.165129967</td>
 <td>1.86362E-05</td>
 <td> </td>
 <td>0.639733409</td>
 <td>0.000729772</td>
 </tr>
 <tr>
 <td>spondin 1, (f-spondin) extracellular matrix protein</td>
 <td>BC020531</td>
 <td>1451342_at</td>
 <td> </td>
 <td>0.168035879</td>
 <td>6.67867E-07</td>
 <td> </td>
 <td>0.821042412</td>
 <td>0.023650765</td>
 </tr>
 <tr>
 <td>RIKEN cDNA A930035E12 gene</td>
 <td>AV348640</td>
 <td>1429906_at</td>
 <td> </td>
 <td>5.9086795</td>
 <td>1.747E-07</td>
 <td> </td>
 <td>1.470383201</td>
 <td>0.104085454</td>
 </tr>
 </tbody>
 <tbody>
 <tr>
 <td>BB247294 RIKEN full-length enriched, 7 days neonate cerebellum Mus musculus cDNA clone A730018G18 3', mRNA sequence.</td>
 <td>BB247294</td>
 <td>1447907_x_at</td>
 <td> </td>
 <td>5.9047494</td>
 <td>1.04931E-05</td>
 <td> </td>
 <td>1.968147585</td>
 <td>0.00010636</td>
 </tr>
 <tr>
 <td>FERM domain containing 3</td>
 <td>BB099015</td>
 <td>1437075_at</td>
 <td> </td>
 <td>5.860216</td>
 <td>0.000345581</td>
 <td> </td>
 <td>2.780297178</td>
 <td>1.83072E-06</td>
 </tr>
 <tr>
 <td>neuronal pentraxin 2 /// hypothetical protein LOC100044234</td>
 <td>NM_016789</td>
 <td>1420720_at</td>
 <td> </td>
 <td>5.7568517</td>
 <td>1.34227E-06</td>
 <td> </td>
 <td>2.652516957</td>
 <td>0.000206279</td>
 </tr>
 <tr>
 <td>Transcribed sequences</td>
 <td>BG076361</td>
 <td>1460101_at</td>
 <td> </td>
 <td>5.657735</td>
 <td>2.5015E-06</td>
 <td> </td>
 <td>1.296248831</td>
 <td>0.22870031</td>
 </tr>
 <tr>
 <td>spondin 1, (f-spondin) extracellular matrix protein</td>
 <td>BC020531</td>
 <td>1424415_s_at</td>
 <td> </td>
 <td>0.17783576</td>
 <td>1.01658E-06</td>
 <td> </td>
 <td>0.836181248</td>
 <td>0.001380141</td>
 </tr>
 <tr>
 <td>calbindin-28K</td>
 <td>BB246032</td>
 <td>1448738_at</td>
 <td> </td>
 <td>0.180317904</td>
 <td>1.35961E-05</td>
 <td> </td>
 <td>0.647334052</td>
 <td>4.12268E-09</td>
 </tr>
 <tr>
 <td>MARCKS-like 1</td>
 <td>AV110584</td>
 <td>1437226_x_at</td>
 <td> </td>
 <td>0.186235935</td>
 <td>1.47067E-06</td>
 <td> </td>
 <td>0.499291387</td>
 <td>2.34984E-08</td>
 </tr>
 <tr>
 <td>matrilin 2</td>
 <td>BB338441</td>
 <td>1455978_a_at</td>
 <td> </td>
 <td>0.187783528</td>
 <td>6.19122E-05</td>
 <td> </td>
 <td>0.8967688</td>
 <td>0.282337853</td>
 </tr>
 <tr>
 <td>matrilin 2</td>
 <td>BC005429</td>
 <td>1419442_at</td>
 <td> </td>
 <td>0.188195795</td>
 <td>0.000105295</td>
 <td> </td>
 <td>0.915528892</td>
 <td>0.35282097</td>
 </tr>
 <tr>
 <td>spondin 1, (f-spondin) extracellular matrix protein</td>
 <td>BQ175871</td>
 <td>1442613_at</td>
 <td> </td>
 <td>0.189956563</td>
 <td>9.41195E-06</td>
 <td> </td>
 <td>0.861033222</td>
 <td>0.1394266</td>
 </tr>
 <tr>
 <td>arrestin 3, retinal</td>
 <td>NM_133205</td>
 <td>1450329_a_at</td>
 <td> </td>
 <td>5.2130346</td>
 <td>2.90599E-05</td>
 <td> </td>
 <td>3.944218329</td>
 <td>1.07437E-07</td>
 </tr>
 <tr>
 <td>RIKEN cDNA A330050F15 gene</td>
 <td>AV325555</td>
 <td>1457558_at</td>
 <td> </td>
 <td>0.19186781</td>
 <td>0.000119342</td>
 <td> </td>
 <td>0.660282035</td>
 <td>2.47342E-05</td>
 </tr>
 <tr>
 <td>contactin 3</td>
 <td>BB559510</td>
 <td>1438628_x_at</td>
 <td> </td>
 <td>0.194404608</td>
 <td>4.08641E-07</td>
 <td> </td>
 <td>0.918742591</td>
 <td>0.022545297</td>
 </tr>
 <tr>
 <td>calbindin-28K</td>
 <td>BB246032</td>
 <td>1417504_at</td>
 <td> </td>
 <td>0.196381321</td>
 <td>2.24182E-05</td>
 <td> </td>
 <td>0.619305124</td>
 <td>3.6222E-06</td>
 </tr>
 <tr>
 <td>gastrin releasing peptide</td>
 <td>BC024515</td>
 <td>1424525_at</td>
 <td> </td>
 <td>4.9436426</td>
 <td>3.00588E-05</td>
 <td> </td>
 <td>2.752845903</td>
 <td>5.72954E-07</td>
 </tr>
 <tr>
 <td>sortilin-related VPS10 domain containing receptor 3</td>
 <td>AK018111</td>
 <td>1425111_at</td>
 <td> </td>
 <td>4.885766</td>
 <td>1.03645E-05</td>
 <td> </td>
 <td>1.29051599</td>
 <td>0.029733649</td>
 </tr>
 <tr>
 <td>dopamine receptor D1A</td>
 <td>BE957273</td>
 <td>1455629_at</td>
 <td> </td>
 <td>4.869493</td>
 <td>3.77525E-05</td>
 <td> </td>
 <td>1.815881979</td>
 <td>0.000516498</td>
 </tr>
 <tr>
 <td>proprotein convertase subtilisin/kexin type 5</td>
 <td>BB241731</td>
 <td>1437339_s_at</td>
 <td> </td>
 <td>0.210528027</td>
 <td>7.83039E-05</td>
 <td> </td>
 <td>0.574126078</td>
 <td>9.15496E-05</td>
 </tr>
 </tbody>
 <tbody>
 <tr>
 <td>interleukin 1 receptor, type I</td>
 <td>NM_008362</td>
 <td>1448950_at</td>
 <td> </td>
 <td>0.210572243</td>
 <td>9.64524E-06</td>
 <td> </td>
 <td>0.241135352</td>
 <td>2.79816E-08</td>
 </tr>
 </tbody>
</table>

dissection of hippocampal dentate gyrus from adult mouse

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

The hippocampus is one of the most widely studied areas in the brain because of its important functional role in memory processing and learning, its remarkable neuronal cell plasticity, and its involvement in epilepsy, neurodegenerative diseases, and psychiatric disorders. The hippocampus is composed of distinct regions; the dentate gyrus, which comprises mainly granule neurons, and Ammon's horn, which comprises mainly pyramidal neurons, and the two regions are connected by both anatomic and functional circuits. Many different mRNAs and proteins are selectively expressed in the dentate gyrus, and the dentate gyrus is a site of adult neurogenesis; that is, new neurons are continually generated in the adult dentate gyrus. To investigate mRNA and protein expression specific to the dentate gyrus, laser capture microdissection is often used. This method has some limitations, however, such as the need for special apparatuses and complicated handling procedures. In this video-recorded protocol, we demonstrate a dissection technique for removing the dentate gyrus from adult mouse under a stereomicroscope. Dentate gyrus samples prepared using this technique are suitable for any assay, including transcriptomic, proteomic, and cell biology analyses. We confirmed that the dissected tissue is dentate gyrus by conducting real-time PCR of dentate gyrus-specific genes, tryptophan 2,3-dioxygenase (TDO2) and desmoplakin (Dsp), and Ammon's horn enriched genes, Meis-related gene 1b (Mrg1b) and TYRO3 protein tyrosine kinase 3 (Tyro3). The mRNA expressions of TDO2 and Dsp in the dentate gyrus samples were detected at obviously higher levels, whereas Mrg1b and Tyro3 were lower levels, than those in the Ammon's horn samples. To demonstrate the advantage of this method, we performed DNA microarray analysis using samples of whole hippocampus and dentate gyrus. The mRNA expression of TDO2 and Dsp, which are expressed selectively in the dentate gyrus, in the whole hippocampus of alpha-CaMKII+/- mice, exhibited 0.037 and 0.10-fold changes compared to that of wild-type mice, respectively. In the isolated dentate gyrus, however, these expressions exhibited 0.011 and 0.021-fold changes compared to that of wild-type mice, demonstrating that gene expression changes in dentate gyrus can be detected with greater sensitivity. Taken together, this convenient and accurate dissection technique can be reliably used for studies focused on the dentate gyrus.

A dissection technique for removal of the dentate gyrus from adult mouse under a stereomicroscope was demonstrated in this video-recorded protocol.

The hippocampus is one of the most widely studied areas in the brain because of its important functional role in memory processing and learning, its ...

The hippocampus is one of the most Widely studied areas in the brain because it's important functional role in memory processing. It's remarkable neuroplasticity and it's involvement in epilepsy, neurodegenerative diseases and psychological disorders. The hippocampus is composed of distinct regions, the DTO Gyrus, CO one, and CS three.The DTO gyrus is a site of other neurogenesis and many researchers may be including you are interested in this region. This video recorded protocol, we show a dissection technique for removing theto gers from adult mouth under sterile microscope. This technique allows us to dissect out and intact whole DTO gels.The technique is very useful because large amount of proteins, mease and neurons from the enter gel samples can be obtained and used for any assay including transcriptomic, proteomic and CE biology analysis. Here's Dr.Gil. He is demonstrating his excellent tend dissection technique in this move.He, I watched a movie and was impressed with how skillfully and smoothly you dissect gyrus. You have the technique of an expert. How many times did you have to practice this technique to reach this level of competence?I had dissect out of vintage GY more than 100 times using this technique, but it probably took me only 20 dissections to reach this level of whiskey. Do you think anybody interested in this technique can acquire this skill? Yes, I do.But you must have good control over your hands and finger. Are there any chips you can give us or things that we should pay attention to when performing this technique? Yes.When removing the dia pepper, it's very important not to injure the hippocampus or surrounding area.Such an injury will make it difficult to identify the boundaries between the tenitis and arm's home. Keeping the boundaries in that and patient is crucial. Thank you.Here.Okay, here we go. Place the brain into HighCo.PVS. Cut the brain along the longitudinal al.Cut those lesions posterior to laden. Place the cellular penia media the other side up and remove the several. This is measured side of hippocampus.You can see the boundary of Ditis and the CS three region. Insert a needle chip into Each side of the ditis and slide the needle along with the septic temporal axis of the hippocampus was isolated.I.

dissection-of-hippocampal-dentate-gyrus-from-adult-mouse

Research

JoVE Journal

Biology

This article outlines procedures for preparing hippocampal slices from rats and transgenic mice for the study of synaptic alterations associated with brain aging and age-related neurodegenerative diseases, such as Alzheimer’s disease.

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

This video demonstrates the recording of rhythmic neuronal network activity, also known as theta oscillations, in an isolated hippocampus. Electrodes are inserted into various layers and spatial positions to detect and analyze theta oscillations. This shows the unique neuronal compositions and the connectivity of the neuronal network across different layers of the hippocampus.

In Vitro Recording of Theta Oscillations in the Mouse Hippocampus

This paper presents a complete methodology to prepare and preserve in vitro acute hippocampal slices from adult mice. This protocol allows the recording of very stable long-lasting long-term potentiation (LTP) for more than 8 hours with a success rate of 95%.

Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation

We describe two methods for visualization and quantification of dendritic arborization in the hippocampus of mouse models: real-time and extended depth of field imaging. While the former method allows sophisticated topographical tracing and quantification of the extent of branching, the latter allows speedy visualization of the dendritic tree.

Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice

A protocol to generate a co-culture system consisting of neurons derived from induced pluripotent stem cells (iPSCs), primary cortical neurons and astrocytes is described. This co-culture system allows detection of the formation of synaptic contacts and circuits between new, iPSC-derived neurons and pre-existing cortical neurons expressing channelrhodopsin-2.

An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System

This method describes a chronic preparation that allows optical access to the hippocampus of living mice. This preparation can be used to perform longitudinal optical imaging of neuronal structural plasticity and activity-evoked cellular plasticity over a period of several weeks.

Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice

This is a protocol for acute slice preparation from adult and aging mouse hippocampi that takes advantage of transcardial perfusion and slice cutting with ice-cold NMDG-aCSF to reduce hypoxic damage to the tissue. The resulting slices stay healthy over many hours, and are suitable for long-term patch-clamp and field-recordings.

Minimizing Hypoxia in Hippocampal Slices from Adult and Aging Mice

This article aims to describe a systematic protocol to obtain horizontal hippocampal brain slices in mice. The objective of this methodology is to preserve the integrity of hippocampal fiber pathways, such as the perforant path and the mossy fiber tract to assess dentate gyrus related neurological processes.

Horizontal Hippocampal Slices of the Mouse Brain

We present a protocol to prepare acute-slices from the dorsal-intermediate hippocampus of mice. We compare this transversal preparation with coronal slicing in terms of quality of recordings and preservation of morphological features of recorded neurons.

Preparation of Acute Slices from Dorsal Hippocampus for Whole-Cell Recording and Neuronal Reconstruction in the Dentate Gyrus of Adult Mice

Here, we demonstrate how to combine transfection of primary hippocampal rodent neurons with live-cell confocal imaging to analyze pathological protein-induced effects on axonal transport and identify mechanistic pathways mediating these effects.

Using Live&#45;Cell Imaging to Measure the Effects of Pathological Proteins on Axonal Transport in Primary Hippocampal Neurons

Learn a precise dissection technique for isolating the dentate gyrus from adult mouse hippocampus using a stereomicroscope. This method enables collection of intact samples for transcriptomic and proteomic analyses.

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

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