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Chapters
- 00:07Title
- 01:07Introduction
- 02:18Preparation of Thick Fresh Tissue Sections
- 05:22Staining the Thick Sections with Tetramers
- 10:11Preparing Thin Sections
- 12:17Acquiring Confocal Microscope Images
- 17:56Representative Images of Simultaneous Visualization by ISTH of Immune Effectors and Targets in Tissue Sections
- 19:50Conclusion
A technique combining in situ tetramer staining and in situ hybridization (ISTH) enables visualization, mapping and analysis of the spatial proximity of virus-specific CD8+ T cells to their virus-infected targets, and determination of the quantitative relationships between these immune effectors and targets to infection outcomes.
Tags
Virus-specific CD8+ T CellsVirus-infected CellsVisualizeIn SituImmune Effector CellsImmune DefensesInfectionSpatial ProximityQuantitative RelationshipsClearanceChronic Persistent InfectionsIn Situ Tetramer StainingIn Situ HybridizationViral-RNA+ CellsBattle Between Immune Defenses And InfectionISTH (in Situ Tetramer Staining And In Situ Hybridization)Mapping Of LocationsE:T RatiosTiming And Magnitude Of The Immune Response
