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Journal
/
Biology
/
Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging
JoVE Journal
Biology
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JoVE Journal
Biology
Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging
Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging
DOI:
10.3791/1581-v
•
12:27 min
•
November 25, 2009
•
Melissa Paulite
,
Zahra Fakhraai
,
Boris B. Akhremitchev
,
Kerstin Mueller
,
Gilbert C. Walker
1
Department of Chemistry
,
University of Toronto
,
2
Department of Chemistry
,
University of Wisconsin
,
3
Department of Chemistry
,
Duke University
Chapters
00:00
Title
00:53
Introduction
01:20
Overview of the Imaging System
02:36
Initial Assembly of the Optical Setup
06:50
Routine Adjustment
09:03
Alignment of the IR Beam
11:17
Representative Results/Outcome
11:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
The assembly of a nearfield infrared microscope for imaging protein aggregates is described.
Tags
Assembly
Tuning
Apertureless Near-field Infrared Microscope
Protein Imaging
Diffraction Limit
Infrared Spectra
Resolution
Components
Protocol
Errors
Instrument Tuning
Amyloid Fibril
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