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JoVE Journal
Biology

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Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
 

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering

Article DOI: 10.3791/1590
October 26th, 2009

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Summary October 26th, 2009

We present protocols for the 3-dimensional (3D) encapsulation of cells within synthetic hydrogels. The encapsulation procedure is outlined for two commonly used methods of crosslinking (michael-type addition and light-initiated free radical mechanisms), as well as a number of techniques for assessing encapsulated cell behavior.

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