Name: Denaturing Gradient Gel Electrophoresis (DGGE) (Video) | JoVE Journal
Uploaded: 2007-02-25T00:00:00.000+00:00
Duration: 10 min 52 s
Description: Learn denaturing gradient gel electrophoresis for profiling bacterial communities in microbial biofilms using 16S rRNA sequences. This method enables researchers to analyze genetic diversity and community dynamics over time.

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denaturing gradient gel electrophoresis (dgge)

Denaturing Gradient Gel Electrophoresis (DGGE)

Okay.Hi, my name is Celeste Peterson and I'm a fellow at Harvard University studying microbial bio film communities. So I am profiling the bacterial communities by looking at their 60 N sequence and how this sequence changes over time in space. And to do that, I'm using DDGE.This is not a normal acry of my well, it needs to be kept at 60 degrees when it's run and the buffer needs to be pumped continuously. So we're gonna warm up the buffer for about an hour until it's at 60 degrees, and then we can run the towel. Now I'm gonna wash this plate with dilute uc solution, peeling around for impurities, andone, light that up.Anything like that can cause regularities. It is important to wipe off all the water. So when I assemble the gel, I'll put that chip on the outside of the gel.So the gel will be in between these two pieces of glass and the chip will be on the outside and it won't affect the gel in between. So now I'm gonna assemble the gel in these clamps and then I squeeze them together and tighten by turning to the right. As with any gel, it's important to make sure that the bottom is flush.To prevent making, we now turn the gel around and put it in here to get sealed with the gask

denaturing-gradient-gel-electrophoresis-dgge

Research

JoVE Journal

Biology

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Learn denaturing gradient gel electrophoresis for profiling bacterial communities in microbial biofilms using 16S rRNA sequences. This method enables researchers to analyze genetic diversity and community dynamics over time.

Denaturing Gradient Gel Electrophoresis (DGGE)

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