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Presynaptically Silent Synapses Studied with Light Microscopy
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Presynaptically Silent Synapses Studied with Light Microscopy

Presynaptically Silent Synapses Studied with Light Microscopy

DOI:
10.3791/1676-v

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11:02 min

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January 04, 2010

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, , , ,
1Department of Psychiatry,Washington University School of Medicine, 2Department of Anatomy,Washington University School of Medicine, 3Department of Neurobiology,Washington University School of Medicine

Chapters

  • 00:00Title
  • 00:40Introduction
  • 01:08Preparation of Hippocampal Cultures
  • 03:12Immunostaining of Silent Synapses
  • 06:56Visualization of Silent Synapses
  • 09:20Representative Silent Synapse Data
  • 10:21Conclusion

Summary

Automatic Translation

Automatic Translation

Glutamatergic synapses can switch from an active mode to a silent mode. We demonstrate that presynaptic activity status in dissociated culture of rodent neurons is visualized using a fixable form of the FM1-43 dye to visualize active synapses and immunostaining with vGluT-1 antibody to visualize all glutamate synapses.

Tags

PresynapticSilent SynapsesLight MicroscopySynaptic PlasticityNervous SystemLearnRememberAdaptabilityNeurotoxicPresynaptic PlasticityDigital SwitchingRelease VesiclesGlutamate NeurotransmitterVisualizing Activity StatusDissociated Cell CulturesRodent HippocampusStaining ProfileStyryl Dye FM1-43Synaptic VesiclesImmunostainingVesicular Glutamate Transporter 1 (vGluT-1)Depolarizing StimuliPresynaptic SilencingElectrical SilencingCAMP Signaling Pathways

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