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Biology

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Presynaptically Silent Synapses Studied with Light Microscopy
 

Presynaptically Silent Synapses Studied with Light Microscopy

Article doi: 10.3791/1676
January 4th, 2010

Summary January 4th, 2010

Glutamatergic synapses can switch from an active mode to a silent mode. We demonstrate that presynaptic activity status in dissociated culture of rodent neurons is visualized using a fixable form of the FM1-43 dye to visualize active synapses and immunostaining with vGluT-1 antibody to visualize all glutamate synapses.

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