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JoVE Journal Biology
Presynaptically Silent Synapses Studied with Light Microscopy
 

Presynaptically Silent Synapses Studied with Light Microscopy

Article doi: 10.3791/1676
January 4th, 2010 •
1, 1, 1, 1, 1,2,3
1Department of Psychiatry, Washington University School of Medicine, 2Department of Anatomy, Washington University School of Medicine, 3Department of Neurobiology, Washington University School of Medicine

Summary
Automatic Translation
January 4th, 2010

Glutamatergic synapses can switch from an active mode to a silent mode. We demonstrate that presynaptic activity status in dissociated culture of rodent neurons is visualized using a fixable form of the FM1-43 dye to visualize active synapses and immunostaining with vGluT-1 antibody to visualize all glutamate synapses.

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