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Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

JoVE Journal
Biology

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JoVE Journal Biology

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

DOI:

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17:03 min

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March 23, 2010

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Arkadiusz W. Kulczyk, Nathan A. Tanner, Joseph J. Loparo, Charles C. Richardson, Antoine M. van Oijen

¹Harvard Medical School

Chapters

00:00Title
00:51Introduction
01:18Preparation of the DNA Replication Template
03:41Bead Preparation
05:28Preparing Functionalized Coverslips
08:31Flow Chamber Assembly
11:34Single-Molecule Replication Experiment
15:41Single-Molecular Replication Data Analysis and Representative Results
16:34Conclusion

Summary

Automatic Translation

We describe a method for observing real time replication of individual DNA molecules mediated by proteins of the bacteriophage replication system.

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