Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos

Erica Andersen; Namrata Asuri; Matthew Clay; Mary Halloran

doi:10.3791/1726

Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells...

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Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos

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10:52 min

February 3, 2010

DOI: 10.3791/1726-v

Erica Andersen^1,2,3, Namrata Asuri^1,2,3, Matthew Clay^2,3,4, Mary Halloran^1,2,3,4

¹Genetics Training Program,University of Wisconsin-Madison, ²Department of Anatomy,University of Wisconsin-Madison, ³Department of Zoology,University of Wisconsin-Madison, ⁴Cell and Molecular Biology Training Program,University of Wisconsin-Madison

This protocol describes imaging of individual neurons or neural crest cells in living zebrafish embryos. This method is used to examine cellular behaviors and actin localization using fluorescence confocal time-lapse microscopy.

To image the behavior of individual cells in vivo plasma DNA containing a cell specific promoter driving expression of a flora four or biosensor is injected into zebrafish embryos. Dynamic changes in effect in distribution are visualized in vivo using a biosensor based on the F effect and binding domain of utrophin. Newly fertilized embryos are lined up along the injection slide and positioned in the corner formed between the top and bottom slides.

DNA encoding fluoro fours or biosensors is injected into the cell. The next day. Embryos are mounted in aros.

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