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Primary Human Bronchial Epithelial Cells Grown from Explants
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Primary Human Bronchial Epithelial Cells Grown from Explants

Primary Human Bronchial Epithelial Cells Grown from Explants

DOI:
10.3791/1789-v

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14:32 min

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March 26, 2010

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, ,
1Medicine, Faculty of Health Sciences,McMaster University

Chapters

  • 00:00Title
  • 00:58Coating and Scratching 100mm Culture Plates
  • 04:58Bronchial Tissue Transplants
  • 05:41Passage of Human Bronchial Epithelial (HBE) Cells
  • 08:42Growing Ciliated HBE Cells on Transwells
  • 11:42Representative Results
  • 13:54Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a detailed method for growing primary human bronchial epithelial cells from explants of human bronchial airway tissue including differentiated growth on an air-liquid interface. This method provides an abundant source of primary cells for investigating the role of the airway epithelium in human lung health and disease.

Tags

Human Bronchial Epithelial CellsCell Models Of DiseaseExcipientsPharmacologic AgentsGrowing Bronchial Epithelial CellsLung SurgeryLung CancerLung Volume Reduction SurgeryEthics ApprovalInformed ConsentSource Of ExplantsPrimary Bronchial Epithelial CellsCulture PlatesCollagenFibronectinBSAScratched AreasCulture MediumIncubator

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