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Primary Human Bronchial Epithelial Cells Grown from Explants
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Primary Human Bronchial Epithelial Cells Grown from Explants

Article DOI: 10.3791/1789-v • 14:32 min • March 26th, 2010
March 26th, 2010 •
1, 1, 1
1Medicine, Faculty of Health Sciences, McMaster University

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Summary
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Here we describe a detailed method for growing primary human bronchial epithelial cells from explants of human bronchial airway tissue including differentiated growth on an air-liquid interface. This method provides an abundant source of primary cells for investigating the role of the airway epithelium in human lung health and disease.

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Human Bronchial Epithelial Cells Cell Models Of Disease Excipients Pharmacologic Agents Growing Bronchial Epithelial Cells Bronchial Airway Tissue Lung Surgery Lung Cancer Lung Volume Reduction Surgery Ethics Approval Informed Consent Source Of Explants Primary Bronchial Epithelial Cells Culture Plates Collagen Fibronectin BSA Scratched Areas Culture Medium Incubator
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