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Neuroscience

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Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes
 

Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes

Article doi: 10.3791/1790
February 15th, 2010 Usage Statistics

Summary February 15th, 2010

FM dyes have been of invaluable help in the understanding of synaptic dynamics. FMs are normally followed under the fluorescent microscope during different stimulation conditions. However, photoconversion of FM dyes combined with electron microscopy allows the visualization of distinct synaptic vesicle pools, among other ultrastructure components, in synaptic boutons.

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