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Biology

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Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons
 

Assaying the Ability of Diffusible Signaling Molecules to Reorient Embryonic Spinal Commissural Axons

Article doi: 10.3791/1853
March 8th, 2010 Usage Statistics

Summary March 8th, 2010

This assay assesses the ability of a signaling molecule, here Bone Morphogenetic Protein 7 (BMP7), to reorient commissural axons. An explant of embryonic dorsal spinal cord is cultured adjacent to an aggregate of COS cells secreting the candidate growth factors. Reoriented commissural axons growing within the explant are visualized by immunohistochemistry.

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