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Time-lapse Imaging of Mitosis After siRNA Transfection
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Time-lapse Imaging of Mitosis After siRNA Transfection

Article DOI: 10.3791/1878 • 08:22 min • June 6th, 2010
June 6th, 2010 •
1, 1, 2
1Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, 2Fluorescence Microscopy Core Facility, University of Utah

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Here we describe a basic protocol to image and quantify the mitotic timing of live mammalian tissue culture cells after siRNA transfection.

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Time-lapse Imaging Mitosis SiRNA Transfection Cellular Organization Chromosome Dynamics Fluorescence Microscopy GFP-tagged Proteins RNAi-based Depletion Mitotic Regulatory Protein Automated Fluorescence Microscope Image Sequences Still-image Montages Movies Quantification Of Mitotic Stages MCherry-tagged Histone H2B Time-lapse Experiment Design
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