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Time-lapse Imaging of Mitosis After siRNA Transfection

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Time-lapse Imaging of Mitosis After siRNA Transfection

Time-lapse Imaging of Mitosis After siRNA Transfection

DOI:

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08:21 min

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June 06, 2010

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Douglas R. Mackay, Katharine S. Ullman, Christopher K. Rodesch

¹Department of Oncological Sciences, Huntsman Cancer Institute,University of Utah, ²Fluorescence Microscopy Core Facility,University of Utah

Chapters

00:00Title
00:45Introduction
01:11siRNA Transfection and Cell Preparation
02:18Microscope Setup and Image Acquisition
05:22Image Processing and Analysis
07:21Effect of Knockdown of Nucleoporin Nup153
07:48Conclusion

Summary

Automatic Translation

Here we describe a basic protocol to image and quantify the mitotic timing of live mammalian tissue culture cells after siRNA transfection.

Tags

Time-lapse Imaging Mitosis SiRNA Transfection Cellular Organization Chromosome Dynamics Fluorescence Microscopy GFP-tagged Proteins RNAi-based Depletion Mitotic Regulatory Protein Automated Fluorescence Microscope Image Sequences Still-image Montages Movies Quantification Of Mitotic Stages MCherry-tagged Histone H2B Time-lapse Experiment Design

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