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JoVE Journal
Immunology and Infection
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Measuring the 50% Haemolytic Complement (CH50) Activity of Serum
 

Measuring the 50% Haemolytic Complement (CH50) Activity of Serum

Article DOI: 10.3791/1923
March 29th, 2010

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Summary March 29th, 2010

The classical pathway is activated by antibody and culminates in target cell lysis. The CH50 assay provides a measure of the complement activity of a serum sample. This video demonstrates the steps involved in determining the CH50 of a serum sample, the calculations and interpreting of results.

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